The objective of the current program is to further our understanding of the molecular mechanisms within the brain that contribute to cardiometabolic control. The current core is designed to provide technical and intellectual support for assessments of energy homeostasis in mouse models employed by all four projects within the program. Through this core, projects will have access to an array of equipment used to assess energy homeostasis including multiplexed systems (Promethion, OxyMax), NMR-based body composition analyzers, bomb calorimeters, metabolic caging systems, respirometers, direct calorimeters, cellular respiration systems, urinalysis/blood analysis systems, etc. In addition, core staff will provide surgical support for all projects, and the PI will provide statistical support and training for all projects. The centralizing of these endpoints into a core will support increased rigor and reproducibility of all metabolic phenotyping data across the program.

Public Health Relevance

The objective of this core is to make available, centralize, optimize, and codify methods employed by all projects across the current program with regard to mouse metabolic phenotyping techniques. This core will provide technical and intellectual support to assess energy flux in mouse models, using methods including multiplexed phenotyping (Promethion, OxyMax) systems, NMR-based body composition, metabolic caging, bomb calorimetry, respirometry, direct calorimetry, and other methods. In addition this core will provide expert rodent surgical services and statistical analysis support services for all projects in the program.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL084207-13
Application #
9977815
Study Section
Special Emphasis Panel (ZHL1)
Program Officer
OH, Youngsuk
Project Start
Project End
Budget Start
2020-07-01
Budget End
2021-06-30
Support Year
13
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
Seoane-Collazo, Patricia; Roa, Juan; Rial-Pensado, Eva et al. (2018) SF1-Specific AMPK?1 Deletion Protects Against Diet-Induced Obesity. Diabetes 67:2213-2226
Schmidt, Eric A; Despas, Fabien; Pavy-Le Traon, Anne et al. (2018) Intracranial Pressure Is a Determinant of Sympathetic Activity. Front Physiol 9:11
Scroggins, Sabrina M; Santillan, Donna A; Lund, Jenna M et al. (2018) Elevated vasopressin in pregnant mice induces T-helper subset alterations consistent with human preeclampsia. Clin Sci (Lond) 132:419-436
Forrester, Steven J; Booz, George W; Sigmund, Curt D et al. (2018) Angiotensin II Signal Transduction: An Update on Mechanisms of Physiology and Pathophysiology. Physiol Rev 98:1627-1738
Sandgren, Jeremy A; Linggonegoro, Danny W; Zhang, Shao Yang et al. (2018) Angiotensin AT1A receptors expressed in vasopressin-producing cells of the supraoptic nucleus contribute to osmotic control of vasopressin. Am J Physiol Regul Integr Comp Physiol 314:R770-R780
Pellegrinelli, Vanessa; Peirce, Vivian J; Howard, Laura et al. (2018) Adipocyte-secreted BMP8b mediates adrenergic-induced remodeling of the neuro-vascular network in adipose tissue. Nat Commun 9:4974
Peng, Hua; Jensen, Dane D; Li, Wencheng et al. (2018) Overexpression of the neuronal human (pro)renin receptor mediates angiotensin II-independent blood pressure regulation in the central nervous system. Am J Physiol Heart Circ Physiol 314:H580-H592
Bell, Balyssa B; Harlan, Shannon M; Morgan, Donald A et al. (2018) Differential contribution of POMC and AgRP neurons to the regulation of regional autonomic nerve activity by leptin. Mol Metab 8:1-12
Sandgren, Jeremy A; Deng, Guorui; Linggonegoro, Danny W et al. (2018) Arginine vasopressin infusion is sufficient to model clinical features of preeclampsia in mice. JCI Insight 3:
Yoon, Young-Sil; Tsai, Wen-Wei; Van de Velde, Sam et al. (2018) cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis. Proc Natl Acad Sci U S A 115:E5289-E5297

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