The Biophysical Instrumentation & Protein Purification Core (BIPPC) will assist in the design and executionof a variety of techniques essential for the proposed projects. The core consists of seven scientists currentlylocated within BBRI with expertise in the areas of protein chemistry and peptide synthesis. The analysis ofprotein structure and protein-protein interactions will be approached using a variety of spectroscopicmethods, analytical ultracentrifugation, calorimetry, and surface plasmon resonance. The core will provideprotein engineering capabilities that will allow the expression of proteins and protein constructs required forstudies proposed in projects 2 and 3. Some studies proposed in this application; for example the FRETstudies in Project 2 will be carried out directly by the principal investigator's group within the core. In suchcases, members of the core will be available to provide support when required such as instrumentmaintenance and calibration, help in labeling, purification and proteolytic digestion of proteins, and trainingnew post-docs and technicians in the correct use of our instrumentation.
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