Cytometry core The key objective of the Cytometry Core is to enhance the overall productivity of the Program by providing a cost-effective, centralized resource for the use of antibody based cellular pre-enrichment, subsequent flow cytometry based analysis and purification, thin and whole/thick frozen sections for 2D and 3D imaging, and quantitative imaging cytometry. The core will provide 1) the 8 parameter flow cytometer that is the workhorse analytical instrument for the majority of flow cytometry experiments proposed in all three projects, 2) a centralized, cost-effective resource for the laser scanning cytometry (LaSC) for quantitative imaging cytometry analysis proposed in all three projects, 3) preparation of thick cryostat tissue sections and 3D confocal imaging, 4) a resource for expert advice and consultation in the design and analysis of complex, multicolor flow cytometry and LaSC experiments including FlowJo software licenses for flow cytometry analysis and iCys cytometric analysis software (CompuCyte) for LaSC analysis, and 5) magnets and reagents for cellular rosetting and magnetic-particle based strategies for pre-enrichment of cells of interest prior to subsequent flow cytometry based analysis and/or sorting. By providing a cost-effective, centralized resource for antibody based cellular pre-enrichment and subsequent cytometry (flow cytometry and LaSC) based analysis and purification, the Cytometry core will help further the Programs goals of understanding the pathways which control the function of transfused bone marrow, cord blood, and peripherally derived cells.

Public Health Relevance

Cytometry Core The goal of the Cytometry core is to provide a cost-effective, centralized resource for the use of antibody based cellular pre-enrichment, subsequent flow cytometry based analysis and purification, thin and whole/thick frozen sections for 2D and 3D imaging, and quantitative imaging cytometry for 2D and 3D analysis. The overall goal of this program is to develop more efficient and effective Transfusion Medicine related strategies for the benefit of the blood transfusion recipient. The proposed studies will lead to significant insight and information that will directly translate into optimization of Transfusion Medicine practices in the peri-transplant setting.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL095489-09
Application #
9681466
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Program Officer
Mondoro, Traci
Project Start
Project End
Budget Start
2019-04-01
Budget End
2020-03-31
Support Year
9
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Boston Children's Hospital
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115
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