The Molecular Biology Core will provide expert and efficient laboratory services to Project 1 (Saper), Project 3 (Scammell), and Project 5 (Chamberiin). The major duties of Core B are management and genotyping of mouse colonies and performing in situ hybridization histochemistry. Core B will also produce new bacterial clones for in situ hybridization and new viral vectors as technology evolves. Core B is designed to optimize efficiency, accuracy, and expertise. The Core will manage large, shared mouse colonies to ensure a reliable and efficient supply of accurately genotyped mice for each Project. The Core also will centralize the performance of complex molecular techniques such as in situ hybridization histochemistry and bacterial culture so researchers in the Projects can focus on the science instead of debugging techniques Our years of experience with these methods maximizes the likelihood that the complicated molecular techniques proposed in this PPG will succeed.

Agency
National Institute of Health (NIH)
Institute
National Heart, Lung, and Blood Institute (NHLBI)
Type
Research Program Projects (P01)
Project #
5P01HL095491-05
Application #
8634593
Study Section
Heart, Lung, and Blood Initial Review Group (HLBP)
Project Start
Project End
Budget Start
2014-03-01
Budget End
2015-02-28
Support Year
5
Fiscal Year
2014
Total Cost
$196,098
Indirect Cost
$83,398
Name
Beth Israel Deaconess Medical Center
Department
Type
DUNS #
071723621
City
Boston
State
MA
Country
United States
Zip Code
02215
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