The overall goal of the present proposal is to analyze the structural relationship between chemically identified synaptic terminals and labeled neurons belonging to the primate spinothalamic tract (STT) system. Emphasis will be placed on synaptic endings containing substance P, enkephalin and serotonin immunoreactivity. The reason for choosing these candidate neurotransmitters/neuromodulators is that each has been implicated either in pain transmission or in pain modulation. We will analyze the STT neuron which has been stained for substance P. A morphological survey will be done of terminal types on proximal and distal dendrites. We will analyze two intracellularly injected STT cells which have been subsequently immunostained for either enkephalin or serotonin. These two cells are now embedded in plastic. They will be reconstructed using a drawing tube. Following reconstruction, the soma and selected dendrites from each neuron will be thin sectioned for EM analysis. We will survey the morphological types of synapses on the soma, proximal and distal dendrites of each cell. We will also identify the morphological types of synapses on STT cells that are associated with SP-, enk- and serotonin immunoreactivity.

Project Start
Project End
Budget Start
Budget End
Support Year
16
Fiscal Year
1990
Total Cost
Indirect Cost
Name
University of Texas Medical Br Galveston
Department
Type
DUNS #
041367053
City
Galveston
State
TX
Country
United States
Zip Code
77555
Carter, Michael W; Johnson, Kathia M; Lee, Jun Yeon et al. (2016) Comparison of Mechanical Allodynia and Recovery of Locomotion and Bladder Function by Different Parameters of Low Thoracic Spinal Contusion Injury in Rats. Korean J Pain 29:86-95
Hammell, D C; Zhang, L P; Ma, F et al. (2016) Transdermal cannabidiol reduces inflammation and pain-related behaviours in a rat model of arthritis. Eur J Pain 20:936-48
Young, E E; Bryant, C D; Lee, S E et al. (2016) Systems genetic and pharmacological analysis identifies candidate genes underlying mechanosensation in the von Frey test. Genes Brain Behav 15:604-15
Yuan, Su-Bo; Ji, Guangchen; Li, Bei et al. (2015) A Wnt5a signaling pathway in the pathogenesis of HIV-1 gp120-induced pain. Pain 156:1311-9
Ji, Guangchen; Li, Zhen; Neugebauer, Volker (2015) Reactive oxygen species mediate visceral pain-related amygdala plasticity and behaviors. Pain 156:825-36
Neugebauer, Volker (2015) Amygdala pain mechanisms. Handb Exp Pharmacol 227:261-84
Hassler, Shayne N; Johnson, Kathia M; Hulsebosch, Claire E (2014) Reactive oxygen species and lipid peroxidation inhibitors reduce mechanical sensitivity in a chronic neuropathic pain model of spinal cord injury in rats. J Neurochem 131:413-7
Ji, Guangchen; Neugebauer, Volker (2014) CB1 augments mGluR5 function in medial prefrontal cortical neurons to inhibit amygdala hyperactivity in an arthritis pain model. Eur J Neurosci 39:455-66
Medina, Georgina; Ji, Guangchen; Gr├ęgoire, St├ęphanie et al. (2014) Nasal application of neuropeptide S inhibits arthritis pain-related behaviors through an action in the amygdala. Mol Pain 10:32
Kiritoshi, Takaki; Sun, Hao; Ren, Wenjie et al. (2013) Modulation of pyramidal cell output in the medial prefrontal cortex by mGluR5 interacting with CB1. Neuropharmacology 66:170-8

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