Classifying neurons into functional subgroups is essential to the understanding of function in the central nervous system which has a large number of heterogeneous populations of neurons. In the somatosensory system, many attempts have been made to classify neurons both in the peripheral and central nervous system. However, none of them satisfactorily represent functions of the somatosensory system. The most important function of the somatosensory system is to transmit various sensory modalities and submodalities of somatic sensation. Therefore, the most useful functional classification of the somatosensory system would be grouping neurons by their potential contribution to sensory modalities. It is hypothesized that objective statistical treatment of the responses of somatosensory neurons to a set of stimuli which reflect sensory modalities will lead us to a new and functionally relevant classification scheme for somatosensory neurons. The goal of the present proposal is to test this hypothesis and thereby establish a new objective means of classifying somatosensory neurons. To achieve this goal, the following specific aims are proposed.
First aim i s to develop a """"""""standard set of stimuli"""""""" to apply to the receptive fields of all the somatosensory neurons whose responses can then be used as the basis for a new classification scheme. And then, aims are directed to perform actual classification of somatosensory neurons of the rat and monkey at 4 different levels: 1) primary afferent neurons; 2) dorsal horn neurons; 3) spinothalamic tract neurons; 4) somatosensory neurons in the thalamus. To achieve these aims, experiments will be performed in anesthetized rats and monkeys. Single cell recordings will be made from somatosensory neurons at the above 4 different levels. Multivariate statistical analysis, cluster analysis, will be performed on responses of neurons to the """"""""standard set of stimuli"""""""" to classify neurons objectively. Achieving these specific aims will undoubtedly lead to a better understanding of sensory processing of somatosensory information in normal as well as in diseased states. This is the long term objective of the present proposal. Establishing an objective and functionally meaningful grouping of somatosensory neurons in normal animals will help us to interpret changes seen in diseased states.
|Carter, Michael W; Johnson, Kathia M; Lee, Jun Yeon et al. (2016) Comparison of Mechanical Allodynia and Recovery of Locomotion and Bladder Function by Different Parameters of Low Thoracic Spinal Contusion Injury in Rats. Korean J Pain 29:86-95|
|Hammell, D C; Zhang, L P; Ma, F et al. (2016) Transdermal cannabidiol reduces inflammation and pain-related behaviours in a rat model of arthritis. Eur J Pain 20:936-48|
|Young, E E; Bryant, C D; Lee, S E et al. (2016) Systems genetic and pharmacological analysis identifies candidate genes underlying mechanosensation in the von Frey test. Genes Brain Behav 15:604-15|
|Yuan, Su-Bo; Ji, Guangchen; Li, Bei et al. (2015) A Wnt5a signaling pathway in the pathogenesis of HIV-1 gp120-induced pain. Pain 156:1311-9|
|Ji, Guangchen; Li, Zhen; Neugebauer, Volker (2015) Reactive oxygen species mediate visceral pain-related amygdala plasticity and behaviors. Pain 156:825-36|
|Neugebauer, Volker (2015) Amygdala pain mechanisms. Handb Exp Pharmacol 227:261-84|
|Hassler, Shayne N; Johnson, Kathia M; Hulsebosch, Claire E (2014) Reactive oxygen species and lipid peroxidation inhibitors reduce mechanical sensitivity in a chronic neuropathic pain model of spinal cord injury in rats. J Neurochem 131:413-7|
|Ji, Guangchen; Neugebauer, Volker (2014) CB1 augments mGluR5 function in medial prefrontal cortical neurons to inhibit amygdala hyperactivity in an arthritis pain model. Eur J Neurosci 39:455-66|
|Medina, Georgina; Ji, Guangchen; Grégoire, Stéphanie et al. (2014) Nasal application of neuropeptide S inhibits arthritis pain-related behaviors through an action in the amygdala. Mol Pain 10:32|
|Kiritoshi, Takaki; Sun, Hao; Ren, Wenjie et al. (2013) Modulation of pyramidal cell output in the medial prefrontal cortex by mGluR5 interacting with CB1. Neuropharmacology 66:170-8|
Showing the most recent 10 out of 585 publications