Abstract

The morphology and tissue culture core will provide histological and tissue culture support for all three projects. Immunohistochemistry for tyrosine hydroxylase will be used as a primary marker for dopaminergic neurons. Quantitation of dopamine neuron numbers in grafted tissue and in the substantia nigra of MPTP treated monkeys will be assessed for each relevant experiment. Computer assisted three-dimensional reconstructions will be made, particularly for attempts at circuit reconstruction using striatal- nigral co-grafts. Additional morphological methods and techniques provided by this core and its personnel are cryopreservation, fluorescence histochemistry, riboprobe in situ hybridization for tyrosine hydroxylase, immunochemistry for a number of other transmitters. Core personnel perform all fetal brain dissections and participate in adult brain dissections and micropunches that rely upon a combined histochemical/biochemical procedure. The tissue culture facility will carry out pharmacologic studies described in Project 1.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS024032-13
Application #
6112267
Study Section
Project Start
1999-05-01
Project End
2002-04-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
13
Fiscal Year
1999
Total Cost
Indirect Cost

  Institution

Name
Yale University
Department
Type
DUNS #
082359691
City
New Haven
State
CT
Country
United States
Zip Code
06520

  Publications

Redmond Jr, D Eugene; Evans, Lawrence (2012) Determination of fetal age by ultrasonography in St. Kitts green monkeys. Am J Primatol 74:433-41
Hurley, P J; Elsworth, J D; Whittaker, M C et al. (2011) Aged monkeys as a partial model for Parkinson's disease. Pharmacol Biochem Behav 99:324-32
Collier, Timothy J; Steece-Collier, Kathy; McGuire, Susan et al. (2003) Cellular models to study dopaminergic injury responses. Ann N Y Acad Sci 991:140-51
Leranth, Csaba; Shanabrough, Marya; Redmond Jr, D Eugene (2002) Gonadal hormones are responsible for maintaining the integrity of spine synapses in the CA1 hippocampal subfield of female nonhuman primates. J Comp Neurol 447:34-42
Redmond Jr, D Eugene (2002) Cellular replacement therapy for Parkinson's disease--where we are today? Neuroscientist 8:457-88
Collier, Timothy J; Sortwell, Caryl E; Elsworth, John D et al. (2002) Embryonic ventral mesencephalic grafts to the substantia nigra of MPTP-treated monkeys: feasibility relevant to multiple-target grafting as a therapy for Parkinson's disease. J Comp Neurol 442:320-30
Elsworth, J D; Taylor, J R; Sladek Jr, J R et al. (2000) Striatal dopaminergic correlates of stable parkinsonism and degree of recovery in old-world primates one year after MPTP treatment. Neuroscience 95:399-408
Leranth, C; Roth, R H; Elsworth, J D et al. (2000) Estrogen is essential for maintaining nigrostriatal dopamine neurons in primates: implications for Parkinson's disease and memory. J Neurosci 20:8604-9
Taylor, J R; Elsworth, J D; Lawrence, M S et al. (1999) Spontaneous blink rates correlate with dopamine levels in the caudate nucleus of MPTP-treated monkeys. Exp Neurol 158:214-20
Lawrence, M S; Foellmer, H G; Elsworth, J D et al. (1999) Inflammatory responses and their impact on beta-galactosidase transgene expression following adenovirus vector delivery to the primate caudate nucleus. Gene Ther 6:1368-79

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