The Tissue Culture Facility provides the necessary equipment and facilities for culture experiments. Specifically, the facility is used for preparing insect primary neuron cultures and for maintaining hybridoma cell lines and insect cell lines. It is well-equipped for the services it provides. The facility is overseen by Dr. Richard Levine and is staffed by one full-time technician, Ms. Turner. She is responsible for maintaining the facility and all common-use reagent stocks. This facility will be used by all 5 PPG projects.

Project Start
2005-07-01
Project End
2007-06-30
Budget Start
2005-07-01
Budget End
2007-06-30
Support Year
15
Fiscal Year
2005
Total Cost
$178,545
Indirect Cost
Name
University of Arizona
Department
Type
DUNS #
806345617
City
Tucson
State
AZ
Country
United States
Zip Code
85721
Srinivasan, Subhashini; Lance, Kimberley; Levine, Richard B (2012) Contribution of EAG to excitability and potassium currents in Drosophila larval motoneurons. J Neurophysiol 107:2660-71
Srinivasan, Subhashini; Lance, Kimberley; Levine, Richard B (2012) Segmental differences in firing properties and potassium currents in Drosophila larval motoneurons. J Neurophysiol 107:1356-65
Mallory, Heather S; Gibson, Nicholas J; Hayashi, Jon H et al. (2011) Direct and glia-mediated effects of GABA on development of central olfactory neurons. Neuron Glia Biol 7:143-61
Schaefer, Jennifer E; Worrell, Jason W; Levine, Richard B (2010) Role of intrinsic properties in Drosophila motoneuron recruitment during fictive crawling. J Neurophysiol 104:1257-66
Gibson, Nicholas J; Tolbert, Leslie P; Oland, Lynne A (2009) Roles of specific membrane lipid domains in EGF receptor activation and cell adhesion molecule stabilization in a developing olfactory system. PLoS One 4:e7222
Sztarker, Julieta; Strausfeld, Nicholas; Andrew, David et al. (2009) Neural organization of first optic neuropils in the littoral crab Hemigrapsus oregonensis and the semiterrestrial species Chasmagnathus granulatus. J Comp Neurol 513:129-50
Strausfeld, Nicholas J; Sinakevitch, Irina; Brown, Sheena M et al. (2009) Ground plan of the insect mushroom body: functional and evolutionary implications. J Comp Neurol 513:265-291
Oland, Lynne A; Biebelhausen, John P; Tolbert, Leslie P (2008) Glial investment of the adult and developing antennal lobe of Drosophila. J Comp Neurol 509:526-50
Hartwig, Cortnie L; Worrell, Jason; Levine, Richard B et al. (2008) Normal dendrite growth in Drosophila motor neurons requires the AP-1 transcription factor. Dev Neurobiol 68:1225-42
Abeytunga, D Thusitha U; Oland, Lynne; Somogyi, Arpad et al. (2008) Structural studies on the neutral glycosphingolipids of Manduca sexta. Bioorg Chem 36:70-6

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