Abstract

The Pathology Core is responsible for all the morphological assessments of various strains of mice injected with Theiler's virus, or immunized with myelin antigens for development of experimental autoimmune encephalomyelitis. The Pathology Core is housed in Dr. Rodriguez's research laboratory. The major focus of the Pathology Core is primarily on plastic-embedded sections embedded in either glycol methacrylate or Araldite. These are the standard assays for assessment of demyelination, remyelination, spinal cord atrophy, and axonal loss. In addition, brain and spinal cord sections are embedded in paraffin for immunocytochemistry and in situ hybridization to detect virus replication. Frozen sections are used for immunofluorescence staining for neurofilament proteins. T cell and cytokine expression and [3H] saxitoxin-labeling to assess axonal integrity.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
1P01NS038468-01A2
Application #
6359229
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
2000-09-01
Project End
2005-08-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2000
Total Cost
$195,164
Indirect Cost

  Institution

Name
Mayo Clinic, Rochester
Department
Type
DUNS #
City
Rochester
State
MN
Country
United States
Zip Code
55905

  Publications

Watzlawik, Jens O; Painter, Meghan M; Wootla, Bharath et al. (2015) A human anti-polysialic acid antibody as a potential treatment to improve function in multiple sclerosis patients. J Nat Sci 1:
Pavelko, Kevin D; Mendez-Fernandez, Yanice; Bell, Michael P et al. (2012) Nonequivalence of classical MHC class I loci in ability to direct effective antiviral immunity. PLoS Pathog 8:e1002541
Mangalam, Ashutosh K; Luckey, David; Giri, Shailendra et al. (2012) Two discreet subsets of CD8 T cells modulate PLP(91-110) induced experimental autoimmune encephalomyelitis in HLA-DR3 transgenic mice. J Autoimmun 38:344-53
Kerkvliet, Jason; Edukulla, Ramakrishna; Rodriguez, Moses (2010) Novel roles of the picornaviral 3D polymerase in viral pathogenesis. Adv Virol 2010:368068
Warrington, Arthur E; Rodriguez, Moses (2010) Method of identifying natural antibodies for remyelination. J Clin Immunol 30 Suppl 1:S50-5
Deb, Chandra; Lafrance-Corey, Reghann G; Zoecklein, Laurie et al. (2009) Demyelinated axons and motor function are protected by genetic deletion of perforin in a mouse model of multiple sclerosis. J Neuropathol Exp Neurol 68:1037-48
Rodriguez, Moses; Warrington, Arthur E; Pease, Larry R (2009) Invited Article: Human natural autoantibodies in the treatment of neurologic disease. Neurology 72:1269-76
Rodriguez, Moses; Zoecklein, Laurie; Papke, Louisa et al. (2009) Tumor necrosis factor alpha is reparative via TNFR2 [corrected] in the hippocampus and via TNFR1 [corrected] in the striatum after virus-induced encephalitis. Brain Pathol 19:12-26
Wright, Brent R; Warrington, Arthur E; Edberg, Dale D et al. (2009) Cellular mechanisms of central nervous system repair by natural autoreactive monoclonal antibodies. Arch Neurol 66:1456-9
Rodriguez, Moses; Zoecklein, Laurie; Kerkvliet, Jason G et al. (2008) Human HLA-DR transgenes protect mice from fatal virus-induced encephalomyelitis and chronic demyelination. J Virol 82:3369-80

Showing the most recent 10 out of 48 publications