The Protein Biochemistry Core will provide support and expertise in protein isolation and analysis for the COBRE research project investigators, as well as other faculty at Sanford Research/USD. The core will provide support and consultation for gel-based proteomic separations, robotic spot excision from polyacrylamide gels, multiplex protein analysis, protein fractionation, semi-preparative and analytical high performance liquid chromatography (HPLC), and screening for protein-protein interactions with the novel BiolD approach. In addition, the core will provide training and maintenance for its shared equipment. The core will also provide assistance in preparation of manuscripts and funding applications as they relate to the services provided. By providing these services in-house, the core will directly interface with the investigators during project design, protocol establishment, troubleshooting and execution as well as organize any further analysis outside of the core. The purpose of this core is to support the goals of its users, namely independent scientists seeking to uncover the mechanisms of human health and disease.

Public Health Relevance

The Protein Biochemistry Core will facilitate the isolation, identification and characterization of proteins for its users. This translates into a more complete understanding of how those proteins function in both health and disease.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM103620-05
Application #
9335894
Study Section
Special Emphasis Panel (ZGM1)
Project Start
Project End
Budget Start
2017-09-01
Budget End
2018-08-31
Support Year
5
Fiscal Year
2017
Total Cost
Indirect Cost
Name
Sanford Research/Usd
Department
Type
DUNS #
050113252
City
Sioux Falls
State
SD
Country
United States
Zip Code
57104
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Anderson, Ruthellen H; Kerkvliet, Jason G; Otta, Jaelin J et al. (2018) Generation of a CLTA reporter human induced pluripotent stem cell line, CRMi001-A-1, using the CRISPR/Cas9 system to monitor endogenous clathrin trafficking. Stem Cell Res 33:95-99
Lucido, Christopher T; Callejas-Valera, Juan L; Colbert, Paul L et al. (2018) ?2-Adrenergic receptor modulates mitochondrial metabolism and disease progression in recurrent/metastatic HPV(+) HNSCC. Oncogenesis 7:81
McKenzie, Casey W; Preston, Claudia C; Finn, Rozzy et al. (2018) Strain-specific differences in brain gene expression in a hydrocephalic mouse model with motile cilia dysfunction. Sci Rep 8:13370
Miszuk, Jacob M; Xu, Tao; Yao, Qingqing et al. (2018) Functionalization of PCL-3D Electrospun Nanofibrous Scaffolds for Improved BMP2-Induced Bone Formation. Appl Mater Today 10:194-202

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