The Clemson Light Imaging Facility (CLIF) will serve as the Imaging and Cell Sorting Core for the EPIC COBRE. The CLIF is a multi-user core facility located in the new Clemson University Life Sciences Facility. The core is under the direction of Dr. Terri Bruce, and houses a suite of Nikon microscopes, a Zeiss LSM 510 confocal microscope, a CytoViva Hyperspectral Imaging System, a Leica DM 750 P polarized light microscope, a Leica DM IL inverted contrasting microscope, a Leica M80 stereomicroscope, a Leica TCS SPE spectral confocal and a Leica SP8X multiphoton spectral confocal microscope. Additionally, the CLIF houses cell counting and sorting equipment, including a Guava EasyCyte HT and a BD Influx FACS cell sorter. The CLIF includes a multi-user specimen preparation laboratory, and a dedicated 20-seat classroom equipped with video conferencing and recording capabilities.
The specific aims of the Imaging and Cell Sorting Core are 1) to enhance the cell sorting capabilities of the Clemson Light Imaging Facility (CLIF); 2) to promote and facilitate the research undertaken by the target PIs of this COBRE; and 3) to promote and facilitate research at Clemson University and the state of South Carolina.
Aim 1 will be addressed by the addition of a BioRad S3 Cell Sorter (funds from CU Provost) and a dedicated cell sorting technician.
Aims 2 &3 will be addressed through providing assistance to target investigators with design and implementation of visualization and cell sorting experiments, providing advanced technique and equipment training, and providing organized workshops on multiphoton/confocal imaging and advanced cell sorting.
The proposed development of CLIF at Clemson University is important for the advancement of research projects that are components of the COBRE as well as those throughout South Carolina. CLIF will provide training, support and access to state-of-the-art microscopes and other cell sorting/cytometry instrumentation.
|Qiu, Yijian; Milanes, Jillian E; Jones, Jessica A et al. (2018) Glucose Signaling Is Important for Nutrient Adaptation during Differentiation of Pleomorphic African Trypanosomes. mSphere 3:|
|Ray, Sunayan S; Wilkinson, Christina L; Paul, Kimberly S (2018) Regulation of Trypanosoma brucei Acetyl Coenzyme A Carboxylase by Environmental Lipids. mSphere 3:|
|Altamirano, Sophie; Simmons, Charles; Kozubowski, Lukasz (2018) Colony and Single Cell Level Analysis of the Heterogeneous Response of Cryptococcus neoformans to Fluconazole. Front Cell Infect Microbiol 8:203|
|Tajielyato, Nayere; Li, Lin; Peng, Yunhui et al. (2018) E-hooks provide guidance and a soft landing for the microtubule binding domain of dynein. Sci Rep 8:13266|
|Kafková, Lucie; Tu, Chengjian; Pazzo, Kyle L et al. (2018) Trypanosoma brucei PRMT1 Is a Nucleic Acid Binding Protein with a Role in Energy Metabolism and the Starvation Stress Response. MBio 9:|
|Ramos-Garcia, Angel A; Shankar, Vijay; Saski, Christopher A et al. (2018) Draft Genome Sequence of the 1,4-Dioxane-Degrading Bacterium Pseudonocardia dioxanivorans BERK-1. Genome Announc 6:|
|Gordhan, Heeren M; Milanes, Jillian E; Qiu, Yijian et al. (2017) A targeted delivery strategy for the development of potent trypanocides. Chem Commun (Camb) 53:8735-8738|
|Di Cristina, Manlio; Dou, Zhicheng; Lunghi, Matteo et al. (2017) Toxoplasma depends on lysosomal consumption of autophagosomes for persistent infection. Nat Microbiol 2:17096|
|Lin, Sheng; Voyton, Charles; Morris, Meredith T et al. (2017) pH regulation in glycosomes of procyclic form Trypanosoma brucei. J Biol Chem 292:7795-7805|
|Flaherty, Daniel P; Harris, Michael T; Schroeder, Chad E et al. (2017) Optimization and Evaluation of Antiparasitic Benzamidobenzoic Acids as Inhibitors of Kinetoplastid Hexokinase?1. ChemMedChem 12:1994-2005|
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