Abstract

The innate immune response to pathogens involves the expression and secretion of inflammatory cytokines that act to facilitate eradication of the infecting pathogen. The dysregulation of cytokine production (chronic inflammation) can have negative effects on the host. Therefore, a fundamental understanding of the molecular mechanisms regulating inflammation is essential for the development of therapeutic approaches for inflammatory disorders. We identify a novel role for the transcription factor GLI3 in regulating cytokine expression in response to TLR-TRIF signaling. GLI3 KO in monocytes induces a phenotype that indicates GLI3 associates with inflammatory pathways in response to TLR-TRIF stimulation. In this proposal, we will further elucidate the role of GLI3 in regulating cytokine expression in monocytes and explore the relevance of this regulation in inflammatory responses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Exploratory Grants (P20)
Project #
5P20GM113131-04
Application #
10176697
Study Section
Special Emphasis Panel (ZGM1)
Program Officer
Bernal, Federico
Project Start
Project End
Budget Start
2020-06-01
Budget End
2021-05-31
Support Year
4
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
University of New Hampshire
Department
Type
DUNS #
111089470
City
Durham
State
NH
Country
United States
Zip Code
03824

  Publications

Mohammadi, Mohammadjavad; Mohammadiarani, Hossein; Shaw, Vincent S et al. (2018) Interplay of cysteine exposure and global protein dynamics in small-molecule recognition by a regulator of G-protein signaling protein. Proteins :
Tooley, Christian A; Gasperoni, Charles H; Marnoto, Sabrina et al. (2018) Evaluation of Metal Oxide Surface Catalysts for the Electrochemical Activation of Amino Acids. Sensors (Basel) 18:
Ghosh, Sujoy; Khan, Niazul I; Tsavalas, John G et al. (2018) Selective Detection of Lysozyme Biomarker Utilizing Large Area Chemical Vapor Deposition-Grown Graphene-Based Field-Effect Transistor. Front Bioeng Biotechnol 6:29
Arral, Mariah L; Halpern, Jeffrey M (2018) Electrochemical Detection of NG-Hydroxy-L-arginine. ECS Trans 85:1163-1169
Charntikov, Sergios; Pittenger, Steven T; Pudiak, Cindy M et al. (2018) The effect of N-acetylcysteine or bupropion on methamphetamine self-administration and methamphetamine-triggered reinstatement of female rats. Neuropharmacology 135:487-495
Morales, Marissa A; Halpern, Jeffrey Mark (2018) Guide to Selecting a Biorecognition Element for Biosensors. Bioconjug Chem 29:3231-3239
Thompson, Garrett; Marnoto, Sabrina; Halpern, Jeffrey M (2017) Proper Controls to Electrochemically Evaluate Carotenoids using ?-Cyclodextrin Modified Surfaces. ECS Trans 80:1177-1187