Abstract

A.
Specific Aims This project will concern the properties and biological applications of peptides that interact with membranes or with the extracellular matrix. We seek new information and new understanding of peptide/lipid interactions and peptide/matrix interactions. We seek to apply some of this information to develop potential new strategies for treating Clostridium infections. Two of our systems of investigation will be drawn from Clostridium, while a third fundamental approach will use a model system to explore peptide folding within a lipid environment.
The specific aims are these: 1. To understand peptide transport across membranes and the preferential use of low M/T peptides by Clostridium difficile for growth and colonization. 2. To elucidate general principles that govern the folding and biological function of membrane-spanning proteins. 3. To understand the mechanisms by which the extracellular matrix is recognized by a collagen-binding domain (CBD) from Clostridium histolyticum, and to develop peptides that could mimic the CBD. Such peptides would then be fused to autocrine/paracrine factors such as growth for the development of a wide range of novel therapeutic agents with potential clinical applications.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
1P20RR015569-01
Application #
6383453
Study Section
Special Emphasis Panel (ZRR1)
Project Start
2000-09-30
Project End
2005-08-31
Budget Start
Budget End
Support Year
1
Fiscal Year
2000
Total Cost
Indirect Cost

  Institution

Name
University of Arkansas at Fayetteville
Department
Type
DUNS #
191429745
City
Fayetteville
State
AR
Country
United States
Zip Code
72701

  Publications

Davis, Julie Eberle; Alghanmi, Arwa; Gundampati, Ravi Kumar et al. (2018) Probing the role of proline -135 on the structure, stability, and cell proliferation activity of human acidic fibroblast growth factor. Arch Biochem Biophys 654:115-125
Kang, Seong W; Jayanthi, Srinivas; Nagarajan, Gurueswar et al. (2018) Identification of avian vasotocin receptor subtype-specific antagonists involved in the stress response of the chicken, Gallus gallus. J Biomol Struct Dyn :1-15
Jayanthi, Srinivas; Gundampati, Ravi Kumar; Kumar, Thallapuranam Krishnaswamy Suresh (2017) Simple and Efficient Purification of Recombinant Proteins Using the Heparin-Binding Affinity Tag. Curr Protoc Protein Sci 90:6.16.1-6.16.13
Prudovsky, Igor; Kacer, Doreen; Davis, Julie et al. (2016) Folding of Fibroblast Growth Factor 1 Is Critical for Its Nonclassical Release. Biochemistry 55:1159-67
Manoj, Kelath Murali; Parashar, Abhinav; Gade, Sudeep K et al. (2016) Functioning of Microsomal Cytochrome P450s: Murburn Concept Explains the Metabolism of Xenobiotics in Hepatocytes. Front Pharmacol 7:161
Yadav, N; Kumar, S; Marlowe, T et al. (2015) Oxidative phosphorylation-dependent regulation of cancer cell apoptosis in response to anticancer agents. Cell Death Dis 6:e1969
Stratford Jr, Robert; Vu, Christopher; Sakon, Joshua et al. (2014) Pharmacokinetics in rats of a long-acting human parathyroid hormone-collagen binding domain peptide construct. J Pharm Sci 103:768-75
Ponnapakkam, T; Katikaneni, R; Sakon, J et al. (2014) Treating osteoporosis by targeting parathyroid hormone to bone. Drug Discov Today 19:204-8
Katikaneni, Ranjitha; Ponnapakkam, Tulasi; Matsushita, Osamu et al. (2014) Treatment and prevention of chemotherapy-induced alopecia with PTH-CBD, a collagen-targeted parathyroid hormone analog, in a non-depilated mouse model. Anticancer Drugs 25:30-8
Katikaneni, Ranjitha; Ponnapakkam, Tulasi; Seymour, Andrew et al. (2014) Parathyroid hormone linked to a collagen binding domain promotes hair growth in a mouse model of chemotherapy-induced alopecia in a dose-dependent manner. Anticancer Drugs 25:819-25

Showing the most recent 10 out of 204 publications