This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The major objective of the proposed research plan is to trace the signal transduction pathways responsible for integrin-mediated upregulation of Bcl-2 transcription and cell survival. I will use cell culture and molecular biology techniques. I hypothesize that integrins mediate cell survival by activating signal transduction pathways that lead to increased Bcl-2 expression. This work has the following specific aims: 1. To investigate the role of known signal transduction proteins in integrin mediated upregulation of bcl-2 expression. 2. To isolate by expression cloning proteins that upregulate bcl-2 transcription (BITs) and determine how they affect integrin-mediated signals. 3. To determine the expression patterns of these BIT proteins. 4. To identify proteins that interact with BITs by yeast two-hybrid analysis. Understanding these pathways will provide the molecular mechanism by which integrins mediated survival signals.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR016453-08
Application #
7959639
Study Section
Special Emphasis Panel (ZRR1-RI-5 (01))
Project Start
2009-06-01
Project End
2010-05-31
Budget Start
2009-06-01
Budget End
2010-05-31
Support Year
8
Fiscal Year
2009
Total Cost
$316,705
Indirect Cost
Name
University of Hawaii
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
965088057
City
Honolulu
State
HI
Country
United States
Zip Code
96822
Jansen, Chad; Speck, Mark; Greineisen, William E et al. (2017) Transcriptional and Functional Plasticity Induced by Chronic Insulin Exposure in a Mast Cell-Like Basophilic Leukemia Cell Model. J Immunobiol 2:
Pomozi, Viola; Brampton, Christopher; Szeri, Flóra et al. (2017) Functional Rescue of ABCC6 Deficiency by 4-Phenylbutyrate Therapy Reduces Dystrophic Calcification in Abcc6-/- Mice. J Invest Dermatol 137:595-602
Baumer, Yvonne; McCurdy, Sara; Weatherby, Tina M et al. (2017) Hyperlipidemia-induced cholesterol crystal production by endothelial cells promotes atherogenesis. Nat Commun 8:1129
Doe, Jinger; Kaindl, Angela M; Jijiwa, Mayumi et al. (2017) PTRH2 gene mutation causes progressive congenital skeletal muscle pathology. Hum Mol Genet 26:1458-1464
Wilcox, Christie L; Headlam, Jasmine L; Doyle, Thomas K et al. (2017) Assessing the Efficacy of First-Aid Measures in Physalia sp. Envenomation, Using Solution- and Blood Agarose-Based Models. Toxins (Basel) 9:
Yanagihara, Angel Anne; Wilcox, Christie L (2017) Cubozoan Sting-Site Seawater Rinse, Scraping, and Ice Can Increase Venom Load: Upending Current First Aid Recommendations. Toxins (Basel) 9:
Doyle, Thomas K; Headlam, Jasmine L; Wilcox, Christie L et al. (2017) Evaluation of Cyanea capillata Sting Management Protocols Using Ex Vivo and In Vitro Envenomation Models. Toxins (Basel) 9:
Yanagihara, Angel A; Wilcox, Christie; King, Rebecca et al. (2016) Experimental Assays to Assess the Efficacy of Vinegar and Other Topical First-Aid Approaches on Cubozoan (Alatina alata) Tentacle Firing and Venom Toxicity. Toxins (Basel) 8:
Hartmann, Bianca; Wai, Timothy; Hu, Hao et al. (2016) Homozygous YME1L1 mutation causes mitochondriopathy with optic atrophy and mitochondrial network fragmentation. Elife 5:
Cabrera-Fuentes, Hector A; Lopez, Mercedes L; McCurdy, Sara et al. (2015) Regulation of monocyte/macrophage polarisation by extracellular RNA. Thromb Haemost 113:473-81

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