This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Results of studies proposed will increase our understanding about the toxic effects chlorophenoxy herbicides and will provide insight in the use of Drosophila for studies on human health. Most of the past year was occupied with buying equipment and supplies and building a workable laboratory space. Experiments were begun in January 2005. Our collaborator Mary Stewart at NDSU showed us the basics for Drosophila culture and techniques used for exposure of flies and embryos to herbicides, and harvesting flies and embryos.
For Specific Aim 1 we will develop a model to study tracheal development of the Drosophila. Experiment 1 will establish the LC 50 of 2,4-D, MCPA and Bromoxynil. The first experiments done with 2,4-D were not successful because all the flies died due to the ethanol used as a solvent. Experiments using a different solvent (3% ethanol, 1 % Tween 80 in water) are currently underway. Also alternative exposure on filter papers is being tested. LC 50 experiments for MCPA and Bromoxynil are underway. For Experiment 2 we study the effect of the model compounds on the life cycle of the flies. Currently experiments are being done to establish a detailed life cycle in our laboratory for normal flies. An incubator that maintains humidity and temperature and day and night cycle is used in these experiments.
For Specific aim 2 we have started experiments to optimize mRNA and protein extraction from Drosophila embryos and of culturing large quantities of Drosophilas. The goals for the next funding period are to finish the experiments of Specific Aim 1 and start the experiments of Aim 2. Currently students are recruited for the research in the summer of 2005. Connections with the tribal college have been made with the goal to easy transition of Native American students from a 2-year college to a 4-year college and increase the participation of Native Americans in biomedical research.
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