This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The Molecular, Imaging, and Analytic Core consists of 2 interactive facilities: A) Molecular and imaging facility, B) RIA and Analytic facility. The molecular and imaging facility has valuable work stations to perform the following activities: DNA work for genotyping of gene-targeted mice using the GeneAmp PCR system, Western blot analysis using Lico's Odyssey system, Stratagene's real-time PCR system and RNA extraction using Qiagen's BioRobot EZ1 and ELISA station for various assays. A separate histology immunohistochemistry work room is dedicated for morphology and quantitive immunohistochemistry. An Olympus fluorescence microscope equipped with a real time digital image capturing system utilizing the Image-Pro Plus analyzing system allows for quantization of the changes in immunostaining. In a separate facility, HPLC separation and analysis of angiotensin peptides combined with RIA provides a means for quantitive analysis of angiotensin peptides and other hormonal factors in plasma, tissues, and urine. For each major piece of equipment, a research technician is trained to provide support to investigators. However, the individual investigators, fellows, and students are also trained in the various techniques. These setups allow investigators to be free from complicated operations and to focus on their research projects. The RIA and analytic facility performs RIA and ELISA analysis of plasma, tissue, and urine samples. Recent emphasis has been on the development of ELISA for human, rat, and mouse angiotensinogen. Our development of an ELISA for human angiotensinogen has led to many requests to collaborate with other investigators on translational projects related to the intrarenal Renin-Angiotensin receptor in essential hypertension, diabetes, and chronic kidney disease. Published studies that utilized our Core facilities are listed under publications.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR017659-09
Application #
8167891
Study Section
Special Emphasis Panel (ZRR1-RI-5 (01))
Project Start
2010-07-01
Project End
2011-06-30
Budget Start
2010-07-01
Budget End
2011-06-30
Support Year
9
Fiscal Year
2010
Total Cost
$223,498
Indirect Cost
Name
Tulane University
Department
Physiology
Type
Schools of Medicine
DUNS #
053785812
City
New Orleans
State
LA
Country
United States
Zip Code
70118
Anderson, Christopher E; Hamm, L Lee; Batuman, Gem et al. (2018) The association of angiogenic factors and chronic kidney disease. BMC Nephrol 19:117
Gonzalez, Alexis A; Zamora, Leonardo; Reyes-Martinez, Cristian et al. (2017) (Pro)renin receptor activation increases profibrotic markers and fibroblast-like phenotype through MAPK-dependent ROS formation in mouse renal collecting duct cells. Clin Exp Pharmacol Physiol 44:1134-1144
Hu, T; Yao, L; Reynolds, K et al. (2016) The effects of a low-carbohydrate diet on appetite: A randomized controlled trial. Nutr Metab Cardiovasc Dis 26:476-88
Hu, Tian; Yao, Lu; Reynolds, Kristi et al. (2016) Adherence to low-carbohydrate and low-fat diets in relation to weight loss and cardiovascular risk factors. Obes Sci Pract 2:24-31
Li, Wencheng; Sullivan, Michelle N; Zhang, Sheng et al. (2015) Intracerebroventricular infusion of the (Pro)renin receptor antagonist PRO20 attenuates deoxycorticosterone acetate-salt-induced hypertension. Hypertension 65:352-61
Gonzalez, Alexis A; Prieto, Minolfa C (2015) Roles of collecting duct renin and (pro)renin receptor in hypertension: mini review. Ther Adv Cardiovasc Dis 9:191-200
Li, Yuwen; Liu, Jiao; Li, Wencheng et al. (2015) p53 Enables metabolic fitness and self-renewal of nephron progenitor cells. Development 142:1228-41
Dobrowolski, Leszek; Kuczeriszka, Marta; Castillo, Alexander et al. (2015) Role of atrial natriuretic peptide in mediating the blood pressure-independent natriuresis elicited by systemic inhibition of nitric oxide. Pflugers Arch 467:833-41
Hu, Tian; Yao, Lu; Reynolds, Kristi et al. (2015) The Effects of a Low-Carbohydrate Diet vs. a Low-Fat Diet on Novel Cardiovascular Risk Factors: A Randomized Controlled Trial. Nutrients 7:7978-94
Gonzalez, Alexis A; Prieto, Minolfa C (2015) Renin and the (pro)renin receptor in the renal collecting duct: Role in the pathogenesis of hypertension. Clin Exp Pharmacol Physiol 42:14-21

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