This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We propose to investigate mechanisms of transepithelial ion transport in mosquito excretory epithelial cells and its control by a peptide hormone ion transport peptide. African malaria mosquito Anopheles gambiae inhabits in fresh water, requiring active absorption of ions through hindgut and anal papillae. The adult mosquito processes a large amount of the liquid food in a short period of time during and after the blood feeding. We hypothesize that ion transport peptide (ITP) is a major player in the control of mosquito osmoregulatory function as it is known for the locust. Basic biology of the ion transport peptide in the An. gambiae will be studied using various molecular and biochemical techniques. We will specifically ask questions when and where the ITP isoforms are expressed and secreted. The activities of the ITP isoforms on malpighian tubules and hindgut will be studied by measuring the rate of water transport, and by electrophysiological methods. Proposed research will provide the knowledge how the endocrine system controls transepithelial ion transport in mosquitoes. Understanding basic vector biology and physiology will provide rational approaches to develop environmentally benign insecticidal compounds that help to control vector-born diseases, improving human health.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR017686-05
Application #
7381867
Study Section
Special Emphasis Panel (ZRR1-RI-A (02))
Project Start
2006-07-01
Project End
2007-06-30
Budget Start
2006-07-01
Budget End
2007-06-30
Support Year
5
Fiscal Year
2006
Total Cost
$111,782
Indirect Cost
Name
Kansas State University
Department
Anatomy/Cell Biology
Type
Schools of Veterinary Medicine
DUNS #
929773554
City
Manhattan
State
KS
Country
United States
Zip Code
66506
Ishiguro, Susumu; Kawabata, Atsushi; Zulbaran-Rojas, Alejandro et al. (2018) Co-treatment with a C1B5 peptide of protein kinase C? and a low dose of gemcitabine strongly attenuated pancreatic cancer growth in mice through T cell activation. Biochem Biophys Res Commun 495:962-968
Kudo, Takayuki; Wangemann, Philine; Marcus, Daniel C (2018) Claudin expression during early postnatal development of the murine cochlea. BMC Physiol 18:1
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Miyazaki, Hiromitsu; Wangemann, Philine; Marcus, Daniel C (2016) The gastric H,K-ATPase in stria vascularis contributes to pH regulation of cochlear endolymph but not to K secretion. BMC Physiol 17:1
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