Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator.
Specific Aim 1, which previously assumed a single proposed secondary structure for MTB Thi C as a translational riboswitch. We now aim to determine secondary structure models for the """"""""ON"""""""" and """"""""OFF"""""""" states of the Thi C riboswitch through SHAPE probing (described below). Combined with transcriptional and translational assays being developed in collaboration with our colleagues at the Hansen's Disease lab, we aim to determine whether MTB Thi C expression is controlled transcriptionally of translationally through the upstream riboswitch sequence.
Specific Aim 2 Our second specific aim is to obtain novel """"""""hit"""""""" compounds with weak affinity for the ON and/or OFF state of the TPP MTB riboswitch RNAs (Thi C and Thi M).
Specific Aim 3 We aim to test the hypothesis that pyrithiamine pyrophosphate (PTPP) can inhibit growth in MTB by binding to the TPP riboswitch.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR020159-07
Application #
8167888
Study Section
Special Emphasis Panel (ZRR1-RI-B (01))
Project Start
2010-05-01
Project End
2011-04-30
Budget Start
2010-05-01
Budget End
2011-04-30
Support Year
7
Fiscal Year
2010
Total Cost
$73,760
Indirect Cost

  Institution

Name
Louisiana State University A&M Col Baton Rouge
Department
Veterinary Sciences
Type
Schools of Veterinary Medicine
DUNS #
075050765
City
Baton Rouge
State
LA
Country
United States
Zip Code
70803

  Publications

Crossland, Nicholas A; Alvarez, Xavier; Embers, Monica E (2018) Late Disseminated Lyme Disease: Associated Pathology and Spirochete Persistence Posttreatment in Rhesus Macaques. Am J Pathol 188:672-682
Cheemarla, Nagarjuna R; Baños-Lara, Ma Del Rocío; Naidu, Shan et al. (2017) Neutrophils regulate the lung inflammatory response via ?? T cell infiltration in an experimental mouse model of human metapneumovirus infection. J Leukoc Biol 101:1383-1392
Cai, S; Batra, S; Del Piero, F et al. (2016) NLRP12 modulates host defense through IL-17A-CXCL1 axis. Mucosal Immunol 9:503-14
Cai, S; Batra, S; Langohr, I et al. (2016) IFN-? induction by neutrophil-derived IL-17A homodimer augments pulmonary antibacterial defense. Mucosal Immunol 9:718-29
Gautam, Uma Shankar; Mehra, Smriti; Kaushal, Deepak (2015) In-Vivo Gene Signatures of Mycobacterium tuberculosis in C3HeB/FeJ Mice. PLoS One 10:e0135208
Mehra, Smriti; Foreman, Taylor W; Didier, Peter J et al. (2015) The DosR Regulon Modulates Adaptive Immunity and Is Essential for Mycobacterium tuberculosis Persistence. Am J Respir Crit Care Med 191:1185-96
Baños-Lara, Ma Del Rocío; Harvey, Lindsey; Mendoza, Alexander et al. (2015) Impact and regulation of lambda interferon response in human metapneumovirus infection. J Virol 89:730-42
Caskey, John R; Embers, Monica E (2015) Persister Development by Borrelia burgdorferi Populations In Vitro. Antimicrob Agents Chemother 59:6288-95
Pornwiroon, Walairat; Bourchookarn, Apichai; Paddock, Christopher D et al. (2015) Immunoproteomic profiling of Rickettsia parkeri and Rickettsia amblyommii. Ticks Tick Borne Dis 6:829-35
Baños-Lara, Ma Del Rocío; Piao, Boyang; Guerrero-Plata, Antonieta (2015) Differential mucin expression by respiratory syncytial virus and human metapneumovirus infection in human epithelial cells. Mediators Inflamm 2015:347292

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