This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.Introduction: Periodontal disease afflicts nearly 30% of the adult population and is a main cause of tooth loss. The susceptibility of the gingival tissues to plaque-induced periodontal disease may be influenced by the aging process. We hypothesize that aging of periodontal tissues causes changes in the expression of proteins critical for inflammation and tissue regeneration. This results in greater disease susceptibility and lower healing potential in aged subjects. To test this hypothesis, biopsies of gingival tissue from young and aged subjects will be used to examine the expression of inflammatory cytokines and growth factors. The results are expected to serve as a basis for developing novel approaches for therapeutic intervention in periodontitis that may lead to improved periodontal therapies and tooth retention. To this end, the following is the progress for year 1. Objective: To prepare a protocol to study the effects of aging on periodontal disease; to set up the laboratory with the necessary equipment; to become educated as a scientific investigator. Methods: Worked with the mentoring team to prepare the proposal for the COBRE and set up the lab; attended COBRE program sessions. Results: The proposal was approved by the External Advisory Committee (EAC) with minor revisions. The grant was subsequently approved by the NIH an amended award notice was recently received. The protocol also obtained approval by the IRB. Lab space was renovated and the laboratory is being set up, with most equipment already in place. Two research associates (100% and 50% effort) were hired. The PI and the associates are optimizing the laboratory techniques necessary for the project. The PI participated in COBRE sponsored activities, such as the monthly journal club, the grant seeker sessions, the comprehensive clinical training course, and progress reports and presentations. Discussion: The PI with constant cooperation with the mentoring team and the EAC have already clarified methodology issues. Pilot studies are being set-up and the research team is preparing to recruit patients.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
5P20RR020160-04
Application #
7610697
Study Section
Special Emphasis Panel (ZRR1-RI-5 (02))
Project Start
2007-08-01
Project End
2008-07-31
Budget Start
2007-08-01
Budget End
2008-07-31
Support Year
4
Fiscal Year
2007
Total Cost
$343,961
Indirect Cost
Name
Louisiana State Univ Hsc New Orleans
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
782627814
City
New Orleans
State
LA
Country
United States
Zip Code
70112
Kher, Swapnil S; Struckhoff, Amanda P; Alberts, Arthur S et al. (2014) A novel role for p115RhoGEF in regulation of epithelial plasticity. PLoS One 9:e85409
Struckhoff, Amanda P; Rana, Manish K; Kher, Swapnil S et al. (2013) PDZ-RhoGEF is essential for CXCR4-driven breast tumor cell motility through spatial regulation of RhoA. J Cell Sci 126:4514-26
Johnson, Clorinda C; Yu, Alika; Lee, Heeje et al. (2012) Development of a contemporary animal model of Candida albicans-associated denture stomatitis using a novel intraoral denture system. Infect Immun 80:1736-43
Rana, Manish K; Worthylake, Rebecca A (2012) Novel mechanism for negatively regulating Rho-kinase (ROCK) signaling through Coronin1B protein in neuregulin 1 (NRG-1)-induced tumor cell motility. J Biol Chem 287:21836-45
Xu, Xiaoming; Wang, Yapin; Liao, Sumei et al. (2012) Synthesis and characterization of antibacterial dental monomers and composites. J Biomed Mater Res B Appl Biomater 100:1151-62
Fan, Yuwei; Nelson, James R; Alvarez, Jason R et al. (2011) Amelogenin-assisted ex vivo remineralization of human enamel: Effects of supersaturation degree and fluoride concentration. Acta Biomater 7:2293-302
Kher, Swapnil S; Worthylake, Rebecca A (2011) Regulation of ROCKII membrane localization through its C-terminus. Exp Cell Res 317:2845-52
Fidel Jr, Paul L; Cutler, Jim E (2011) Prospects for development of a vaccine to prevent and control vaginal candidiasis. Curr Infect Dis Rep 13:102-7
Harriott, Melphine M; Noverr, Mairi C (2011) Importance of Candida-bacterial polymicrobial biofilms in disease. Trends Microbiol 19:557-63
Lee, H; Yu, A; Johnson, C C et al. (2011) Fabrication of a multi-applicable removable intraoral denture system for rodent research. J Oral Rehabil 38:686-90

Showing the most recent 10 out of 26 publications