This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The genotyping core will be based on the second floor of the BBSRB. At present the core is still being set up. We have developed the PCR-based methodology for genotype analysis and have optimized the DNA preparation protocols. All of the technologies have been tested on a small scale (~50 animals). The equipment, heat blocks, Robocycler PCR machine, repeating pipette sets, and large format agarose electrophoresis apparati, are in place. A detailed set of user instructions are being written to describe what services are to be provided and what the user needs to supply the core in terms of sample, PCR primer sets, and reaction conditions. An advertisement for a technician to run the facility has been circulated and applicants are being interviewed. The technician will handle the mouse genotyping and antibody production.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Exploratory Grants (P20)
Project #
2P20RR020171-06
Application #
7960500
Study Section
Special Emphasis Panel (ZRR1-RI-B (01))
Project Start
2009-09-01
Project End
2010-06-30
Budget Start
2009-09-01
Budget End
2010-06-30
Support Year
6
Fiscal Year
2009
Total Cost
$47,458
Indirect Cost
Name
University of Kentucky
Department
Biochemistry
Type
Schools of Medicine
DUNS #
939017877
City
Lexington
State
KY
Country
United States
Zip Code
40506
Stenslik, M J; Evans, A; Pomerleau, F et al. (2018) Methodology and effects of repeated intranasal delivery of DNSP-11 in awake Rhesus macaques. J Neurosci Methods 303:30-40
Frasinyuk, Mykhaylo S; Zhang, Wen; Wyrebek, Przemyslaw et al. (2017) Developing antineoplastic agents that target peroxisomal enzymes: cytisine-linked isoflavonoids as inhibitors of hydroxysteroid 17-beta-dehydrogenase-4 (HSD17B4). Org Biomol Chem 15:7623-7629
Shrestha, Sanjib K; Kril, Liliia M; Green, Keith D et al. (2017) Bis(N-amidinohydrazones) and N-(amidino)-N'-aryl-bishydrazones: New classes of antibacterial/antifungal agents. Bioorg Med Chem 25:58-66
Cifuentes-Muñoz, Nicolás; Sun, Weina; Ray, Greeshma et al. (2017) Mutations in the Transmembrane Domain and Cytoplasmic Tail of Hendra Virus Fusion Protein Disrupt Virus-Like-Particle Assembly. J Virol 91:
Burikhanov, Ravshan; Hebbar, Nikhil; Noothi, Sunil K et al. (2017) Chloroquine-Inducible Par-4 Secretion Is Essential for Tumor Cell Apoptosis and Inhibition of Metastasis. Cell Rep 18:508-519
Kenlan, Dasha E; Rychahou, Piotr; Sviripa, Vitaliy M et al. (2017) Fluorinated N,N'-Diarylureas As Novel Therapeutic Agents Against Cancer Stem Cells. Mol Cancer Ther 16:831-837
Klimyte, Edita M; Smith, Stacy E; Oreste, Pasqua et al. (2016) Inhibition of Human Metapneumovirus Binding to Heparan Sulfate Blocks Infection in Human Lung Cells and Airway Tissues. J Virol 90:9237-50
Edgar, Rebecca J; Chen, Jing; Kant, Sashi et al. (2016) SpyB, a Small Heme-Binding Protein, Affects the Composition of the Cell Wall in Streptococcus pyogenes. Front Cell Infect Microbiol 6:126
Matveeva, Elena; Maiorano, John; Zhang, Qingyang et al. (2016) Involvement of PARP1 in the regulation of alternative splicing. Cell Discov 2:15046
Emanuelle, Shane; Brewer, M Kathryn; Meekins, David A et al. (2016) Unique carbohydrate binding platforms employed by the glucan phosphatases. Cell Mol Life Sci 73:2765-2778

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