The Gene Expression Vector Core (GEVC) will provide Nathan Shock Center-affiliated investigators with state-of-the-art molecular biological resources that facilitate their studies of normal aging and pathophysiology of age-related disease. The GEVC will assist in the development and production of gene transfer reagents based on the herpes simplex virus- based amplicon vectors. These are suitable for in vitro and in vivo gene transfer studies. In addition the GEVC will perform quantitative nucleic acid measurements using """"""""real-time"""""""" PCR. While the amplicon vector has been shown to be a very effective vehicle for the delivery of genetic material to numerous tissue and cell types of several animal species, its accessibility to investigators in the aging community will facilitate existing studies and enable new ones. Similarly, the """"""""real-time"""""""" quantitative PCR technology has provided to be a reproducible and high throughput method for quantitation of specific nucleic acid sequences (RNA or DNA) from cells and tissues. However, due to the large expense related to initial equipment costs, most investigators are unable to utilize this valuable technology. By taking advantage of the expertise and facilities that already exists at the University of Rochester, the GEVC seeks to provide both amplicon vector-based gene transfer and quantitative PCR technologies to Shock Center investigators locally, regionally and nationally and other researchers pursuing aging-related problems. The GEVC will be housed on the first floor of the newly constructed Arthur Kornberg medical Research Building within the Center for Aging and Developmental Biology. The GEVC will be directed by Dr. William J. Bowers and co-directed by Dr. Howard J. Federoff and, while day-to-day core activities will be performed by a laboratory technical associate. The GEVC scientific oversight committee will consist of the co-directors and Dr. John Olschowka (quantitative PCR consultant).

National Institute of Health (NIH)
National Institute on Aging (NIA)
Center Core Grants (P30)
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Special Emphasis Panel (ZAG1-PKN-2 (M1))
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University of Rochester
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Cox, Christopher; Matheson, Matthew (2014) A comparison of the generalized gamma and exponentiated Weibull distributions. Stat Med 33:3772-80
Kravets, Anatoliy; Qin, Hong; Ahmad, Ausaf et al. (2010) Widespread occurrence of dosage compensation in Candida albicans. PLoS One 5:e10856
Qin, Hong; Lu, Meng; Goldfarb, David S (2008) Genomic instability is associated with natural life span variation in Saccharomyces cerevisiae. PLoS One 3:e2670
Welle, Stephen; Tawil, Rabi; Thornton, Charles A (2008) Sex-related differences in gene expression in human skeletal muscle. PLoS One 3:e1385
Wu, Qiuqian; Kim, Kyung-Ok; Sampson, Erik R et al. (2008) Induction of an osteoarthritis-like phenotype and degradation of phosphorylated Smad3 by Smurf2 in transgenic mice. Arthritis Rheum 58:3132-44
Burris, Clark A; de Silva, Suresh; Narrow, Wade C et al. (2008) Hexamethylene bisacetamide leads to reduced helper virus-free HSV-1 amplicon expression titers via suppression of ICP0. J Gene Med 10:152-64
Lim, Kuei-Cheng; Tyler, Carolyn M; Lim, Seung T et al. (2007) Proteolytic processing of proNGF is necessary for mature NGF regulated secretion from neurons. Biochem Biophys Res Commun 361:599-604
Miller, Renee M; Kiser, Gretchen L; Kaysser-Kranich, T M et al. (2006) Robust dysregulation of gene expression in substantia nigra and striatum in Parkinson's disease. Neurobiol Dis 21:305-13
Baudry, E; Desmadril, M; Werren, J H (2006) Rapid adaptive evolution of the tumor suppressor gene Pten in an insect lineage. J Mol Evol 62:738-44
Kannanayakal, Theresa J; Tao, Haiyang; Vandre, Dale D et al. (2006) Casein kinase-1 isoforms differentially associate with neurofibrillary and granulovacuolar degeneration lesions. Acta Neuropathol 111:413-21

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