Abstract

The Basic Science Core (BSC;Core D) will enhance basic and laboratory-based HIV/AIDS research by CFAR members, by providing access to cutting-edge instrumentation and technologies, and the necessary support, education and training to enable efficient use of these resources. The services provided by Core D will create added value by addressing resource gaps on campus, and by providing CFAR SWG members with access to enhanced services for chemical biology and drug development. Over the funding period of our D-CFAR, Core D has been highly successful in leveraging new equipment valued at over $2M (a 18:1 ROI), and in helping to create a new strategic partnership between UR and Temple University's Moulder Center for Drug Discovery Research. The Core has also developed a highly proactive, user-oriented, approach that has allowed it to serve almost half (42%) of the total DCFAR membership. Building on this solid foundation, five Aims are proposed.
Aim 1 will provide cutting-edge tools and standardized reagents for virology, immunology, and molecular biology of HIV-1, including recombinant Tat for our CNS Reservoirs/Aging SWG, and a new FPLC resource for our RNA Biology SWG.
Aim 2 will provide expertise and resources for chemical and structural biology, including full-service medicinal chemistry resources and a new pilot program with the Moulder Center, of value to both SWGs.
Aim 3 will provide access to next-generation flow cytometry, including imaging- and mass- cytometry. Finally, Aim 4 will provide training and education to promote the use of new tools, and Aim 5 will integrate Core D services with other Cores and institutional resources - thus ensuring comprehensive attention to investigator needs.

Public Health Relevance

; The Basic Science Core (BSC;Core D) will enhance basic and laboratory-based HIV/AIDS research by CFAR members, by providing access to cutting-edge instrumentation and technologies, and the necessary support, education and training to enable efficient use of these resources.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Allergy and Infectious Diseases (NIAID)
Type
Center Core Grants (P30)
Project #
5P30AI078498-07
Application #
8712927
Study Section
Special Emphasis Panel (ZAI1-UKS-A)
Project Start
Project End
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
7
Fiscal Year
2014
Total Cost
$234,863
Indirect Cost
$81,858

  Institution

Name
Case Western Reserve University
Department
Type
DUNS #
041294109
City
Rochester
State
NY
Country
United States
Zip Code
14627

  Publications

Nunn, Amy; Parker, Sharon; McCoy, Katryna et al. (2018) African American Clergy Perspectives About the HIV Care Continuum: Results From a Qualitative Study in Jackson, Mississippi. Ethn Dis 28:85-92
Sillman, Brady; Bade, Aditya N; Dash, Prasanta K et al. (2018) Creation of a long-acting nanoformulated dolutegravir. Nat Commun 9:443
Ogunbajo, Adedotun; Kershaw, Trace; Kushwaha, Sameer et al. (2018) Barriers, Motivators, and Facilitators to Engagement in HIV Care Among HIV-Infected Ghanaian Men Who have Sex with Men (MSM). AIDS Behav 22:829-839
Piekna-Przybylska, Dorota; Maggirwar, Sanjay B (2018) CD4+ memory T cells infected with latent HIV-1 are susceptible to drugs targeting telomeres. Cell Cycle 17:2187-2203
Ocque, Andrew J; Hagler, Colleen E; Morse, Gene D et al. (2018) Development and validation of an LC-MS/MS assay for tenofovir and tenofovir alafenamide in human plasma and cerebrospinal fluid. J Pharm Biomed Anal 156:163-169
McMillan, JoEllyn; Szlachetka, Adam; Slack, Lara et al. (2018) Pharmacokinetics of a Long-Acting Nanoformulated Dolutegravir Prodrug in Rhesus Macaques. Antimicrob Agents Chemother 62:
Piekna-Przybylska, Dorota; Nagumotu, Kavyasri; Reid, Danielle M et al. (2018) HIV-1 infection renders brain vascular pericytes susceptible to the extracellular glutamate. J Neurovirol :
Braksmajer, Amy; Simmons, Janie; Aidala, Angela et al. (2018) Effects of Discrimination on HIV-Related Symptoms in Heterosexual Men of Color. Am J Mens Health 12:1855-1863
Loelius, Shannon G; Lannan, Katie L; Blumberg, Neil et al. (2018) The HIV protease inhibitor, ritonavir, dysregulates human platelet function in vitro. Thromb Res 169:96-104
Loelius, Shannon G; Spinelli, Sherry L; Lannan, Katie L et al. (2018) In Vitro Methods to Characterize the Effects of Tobacco and Nontobacco Products on Human Platelet Function. Curr Protoc Toxicol 76:e46

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