In contrast to most mammalian organs, many cellular elements of normal human skin can now be cultured. Techniques to culture human keratinocytes (first evolved by HSDRC member Dr. Green), dermal fibroblasts, melanocytes, and microvascular endothelial cells are now standardized, and most of these cells can be obtained from at least one commercial vendor (e.g., Clonetics). Each cell type requires different medium, however, and it is impractical for one laboratory to grow all such cells. Furthermore, unless study of one of these individual cell in a consistent and detailed fashion, it is impractical to initiate the culture of such cells on a regular basis. The main goal of the Cell Culture core is to facilitate experimental use of freshly cultured skin cells, of both human and murine origin. With regards to murine cells, particular emphasis will be placed on the culturing of transgenic-derived keratinocytes, melanocytes, dermal fibroblasts and endothelial cells.
The specific aims of the proposal are to provide the following services: 1) Procurement of human and murine skin. 2) Special media preparation for specific cell types. 3) Isolation and cultivation of skin-derived primary cells, including large scale preparations for biochemical studies (such as RNA and cDNA library preparation; cytokine and enzyme purifications, etc.). 4) Training in the in vitro isolation of cellular elements of skin, such that investigators appropriately trained and having access to appropriate media can initiate and maintain cell cultures in their laboratories.

Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Brigham and Women's Hospital
United States
Zip Code
Tian, Tian; Jin, Michelle Qiushuang; Dubin, Krista et al. (2017) IL-1R Type 1-Deficient Mice Demonstrate an Impaired Host Immune Response against Cutaneous Vaccinia Virus Infection. J Immunol 198:4341-4351
Pan, Youdong; Tian, Tian; Park, Chang Ook et al. (2017) Survival of tissue-resident memory T cells requires exogenous lipid uptake and metabolism. Nature 543:252-256
Volpicelli, Elgida R; Lezcano, Cecilia; Zhan, Qian et al. (2014) The multidrug-resistance transporter ABCB5 is expressed in human placenta. Int J Gynecol Pathol 33:45-51
Guenova, Emmanuella; Watanabe, Rei; Teague, Jessica E et al. (2013) TH2 cytokines from malignant cells suppress TH1 responses and enforce a global TH2 bias in leukemic cutaneous T-cell lymphoma. Clin Cancer Res 19:3755-63
Majewska-Szczepanik, Monika; Paust, Silke; von Andrian, Ulrich H et al. (2013) Natural killer cell-mediated contact sensitivity develops rapidly and depends on interferon-?, interferon-? and interleukin-12. Immunology 140:98-110
Zadran, Sohila; McMickle, Robert; Shackelford, David et al. (2013) Monitoring extra-vascular migratory metastasis (EVMM) of migrating cancer cells using an in vitro co-culture system. Protoc exch 2013:
Burkhardt, Ute E; Hainz, Ursula; Stevenson, Kristen et al. (2013) Autologous CLL cell vaccination early after transplant induces leukemia-specific T cells. J Clin Invest 123:3756-65
Dowlatshahi, Mitra; Huang, Victor; Gehad, Ahmed E et al. (2013) Tumor-specific T cells in human Merkel cell carcinomas: a possible role for Tregs and T-cell exhaustion in reducing T-cell responses. J Invest Dermatol 133:1879-89
Seneschal, Julien; Clark, Rachael A; Gehad, Ahmed et al. (2012) Human epidermal Langerhans cells maintain immune homeostasis in skin by activating skin resident regulatory T cells. Immunity 36:873-84
Girouard, Sasha D; Laga, Alvaro C; Mihm, Martin C et al. (2012) SOX2 contributes to melanoma cell invasion. Lab Invest 92:362-70

Showing the most recent 10 out of 113 publications