The Flow Cytometry Core Facility provides a broad range of applications to Rheumatic Disease Core Center? Members including but not limited to the following: light scatter measurements assessing cell volume and? structural differences; single and multiple intracellular and/or surface immunofluorescence measurements;? DNA ploidy measurements and cell cycle analysis; functional measurements of cellular calcium and? potassium flux and oxidative burst; detection and quantitation of apoptotic cell death, incident polarization? study; cell sorting on any or a combination of several analytical criteria.? The Flow Cytometry Core Facility is currently located in the University of Michigan's Cancer and Geriatrics? Center's building and has been in continuous operation under the direction of Dr. Robert Todd since October? 1984. Investigators deliver pre-processed samples to the Core for flow cytometric analysis and/or cell? sorting. Instruments are operated by trained flow cytometry operators (4.4 FTEs). Core instrumentation? includes a BD Biosciences FACSVantage SE multilaser high-speed cell sorter, two BD Biosciences? FACSDiVa multilaser high-speed cell sorters, a BD Biosciences FACSAria multilaser high-speed cell sorter,? a BD Biosciences FACSCalibur multilaser analyzer and a Beckman-Coulter EPICS XL analyzer. UM-RDCC members (28 of 257) account for approximately 35% of total use of the core.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR048310-07
Application #
7483080
Study Section
Special Emphasis Panel (ZAR1)
Project Start
2007-08-01
Project End
2011-07-31
Budget Start
2007-08-01
Budget End
2008-07-31
Support Year
7
Fiscal Year
2007
Total Cost
$66,416
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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