Abstract

The Flow Cytometry Core Facility provides a broad range of applications to Rheumatic Disease Core Center? Members including but not limited to the following: light scatter measurements assessing cell volume and? structural differences; single and multiple intracellular and/or surface immunofluorescence measurements;? DNA ploidy measurements and cell cycle analysis; functional measurements of cellular calcium and? potassium flux and oxidative burst; detection and quantitation of apoptotic cell death, incident polarization? study; cell sorting on any or a combination of several analytical criteria.? The Flow Cytometry Core Facility is currently located in the University of Michigan's Cancer and Geriatrics? Center's building and has been in continuous operation under the direction of Dr. Robert Todd since October? 1984. Investigators deliver pre-processed samples to the Core for flow cytometric analysis and/or cell? sorting. Instruments are operated by trained flow cytometry operators (4.4 FTEs). Core instrumentation? includes a BD Biosciences FACSVantage SE multilaser high-speed cell sorter, two BD Biosciences? FACSDiVa multilaser high-speed cell sorters, a BD Biosciences FACSAria multilaser high-speed cell sorter,? a BD Biosciences FACSCalibur multilaser analyzer and a Beckman-Coulter EPICS XL analyzer. UM-RDCC members (28 of 257) account for approximately 35% of total use of the core.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR048310-07
Application #
7483080
Study Section
Special Emphasis Panel (ZAR1)
Project Start
2007-08-01
Project End
2011-07-31
Budget Start
2007-08-01
Budget End
2008-07-31
Support Year
7
Fiscal Year
2007
Total Cost
$66,416
Indirect Cost

  Institution

Name
University of Michigan Ann Arbor
Department
Type
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109

  Publications

Nair, Thankam S; Kommareddi, Pavan K; Galano, Maria M et al. (2016) SLC44A2 single nucleotide polymorphisms, isoforms, and expression: Association with severity of Meniere's disease? Genomics 108:201-208
Delaney, Colin; Garg, Sanjay K; Yung, Raymond (2015) Analysis of DNA Methylation by Pyrosequencing. Methods Mol Biol 1343:249-64
Morgan, Rachel; Endres, Judith; Behbahani-Nejad, Nilofar et al. (2015) Expression and function of aminopeptidase N/CD13 produced by fibroblast-like synoviocytes in rheumatoid arthritis: role of CD13 in chemotaxis of cytokine-activated T cells independent of enzymatic activity. Arthritis Rheumatol 67:74-85
McDade, Joel R; Archambeau, Ashley; Michele, Daniel E (2014) Rapid actin-cytoskeleton-dependent recruitment of plasma membrane-derived dysferlin at wounds is critical for muscle membrane repair. FASEB J 28:3660-70
Isozaki, Takeo; Amin, M Asif; Arbab, Ali S et al. (2014) Inhibitor of DNA binding 1 as a secreted angiogenic transcription factor in rheumatoid arthritis. Arthritis Res Ther 16:R68
Kulpa, Deanna A; Del Cid, Natasha; Peterson, Kirsten A et al. (2013) Adaptor protein 1 promotes cross-presentation through the same tyrosine signal in major histocompatibility complex class I as that targeted by HIV-1. J Virol 87:8085-98
Saha, Anjan K; Kappes, Ferdinand; Mundade, Amruta et al. (2013) Intercellular trafficking of the nuclear oncoprotein DEK. Proc Natl Acad Sci U S A 110:6847-52
Kahlenberg, J Michelle; Carmona-Rivera, Carmelo; Smith, Carolyne K et al. (2013) Neutrophil extracellular trap-associated protein activation of the NLRP3 inflammasome is enhanced in lupus macrophages. J Immunol 190:1217-26
Mor-Vaknin, Nirit; Legendre, Maureen; Yu, Yue et al. (2013) Murine colitis is mediated by vimentin. Sci Rep 3:1045
Fu, Jiaqi; Ling, Song; Liu, Ying et al. (2013) A small shared epitope-mimetic compound potently accelerates osteoclast-mediated bone damage in autoimmune arthritis. J Immunol 191:2096-103

Showing the most recent 10 out of 88 publications