CRYSTALLOGRAPHY AND COMPUTATIONAL BIOSCIENCES SHARED RESOURCE Project Summary The Crystallography and Computational Biosciences Shared Resource (CCBSR) is a highly-specialized Resource that provides Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) members with access to expertise, consultation, and state-of-the-art structural and computational biology approaches. The resulting data enhance ongoing, peer-reviewed projects and new funding applications. The CCBSR's scientific importance centers on the need to understand the structural basis for protein-protein, protein-DNA/RNA, and protein-ligand/drug interactions; and how dynamics affect these interactions. The use of the CCBSR has steadily increased, as have the depth and breadth of projects. From 2011?2015, the Co-Directors have worked with 27 laboratories from 11 departments (74% WFBCCC members; all with peer-reviewed funding) to publish 36 manuscripts, solve 9 new structures, perform experiments for 18 existing and newly-funded grants, and collect preliminary data for new funding applications. The CCBSR has three co-Directors (W. Todd Lowther, Ph.D.; Thomas Hollis, Ph.D.; Fred Salsbury, Ph.D.), each with different areas of scientific, experimental, and computational expertise. Therefore, after initial consultation, one or more of the Co-Directors assists the WFBCCC member with the development of their project, matching the project to the expertise of a specific Co- Director.
The Specific Aims of the CCBSR are to: 1) Determine the appropriate crystallography and/or computational approach for structural analysis of important biomolecules in cancer research; 2) Conduct initial crystallization trials or simulations to generate preliminary data for grant applications; and 3) Provide facilities and expertise for funded projects to determine the structure of important biomolecules in cancer research. The crystallography component is managed by the WFBCCC but also has institutional funding. The Co-Directors attend the quarterly WFBCCC Shared Resources meeting, which includes discussions on activity, best practices, enhanced capabilities, and administrative management techniques. These meetings provide an overview of operations, finances, and utilization, and facilitate regular evaluation by WFBCCC leadership.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA012197-45
Application #
9848533
Study Section
Subcommittee H - Clinical Groups (NCI)
Project Start
Project End
Budget Start
2020-02-01
Budget End
2021-01-31
Support Year
45
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Wake Forest University Health Sciences
Department
Type
DUNS #
937727907
City
Winston-Salem
State
NC
Country
United States
Zip Code
27157
Farris, Michael; McTyre, Emory R; Okoukoni, Catherine et al. (2018) Cortical Thinning and Structural Bone Changes in Non-Human Primates after Single-Fraction Whole-Chest Irradiation. Radiat Res 190:63-71
Bolduc, Jesalyn A; Nelson, Kimberly J; Haynes, Alexina C et al. (2018) Novel hyperoxidation resistance motifs in 2-Cys peroxiredoxins. J Biol Chem 293:11901-11912
Garland, Mary; Addis, Dylan; Russell, Greg et al. (2018) The Effect of Regional Anesthesia on Oncologic Outcomes after Resection of Colorectal Hepatic Metastases. Am Surg 84:e29-32
Petty, W Jeffrey; Urbanic, James J; Ahmed, Tamjeed et al. (2018) Long-Term Outcomes of a Phase 2 Trial of Chemotherapy With Consolidative Radiation Therapy for Oligometastatic Non-Small Cell Lung Cancer. Int J Radiat Oncol Biol Phys 102:527-535
Paek, M-S; Nightingale, C L; Tooze, J A et al. (2018) Contextual and stress process factors associated with head and neck cancer caregivers' physical and psychological well-being. Eur J Cancer Care (Engl) 27:e12833
Dutta, Samrat; Rivetti, Claudio; Gassman, Natalie R et al. (2018) Analysis of single, cisplatin-induced DNA bends by atomic force microscopy and simulations. J Mol Recognit 31:e2731
Pardee, Timothy S; Anderson, Rebecca G; Pladna, Kristin M et al. (2018) A Phase I Study of CPI-613 in Combination with High-Dose Cytarabine and Mitoxantrone for Relapsed or Refractory Acute Myeloid Leukemia. Clin Cancer Res 24:2060-2073
Atkins, Hannah M; Appt, Susan E; Taylor, Robert N et al. (2018) Systemic Iron Deficiency in a Nonhuman Primate Model of Endometriosis. Comp Med 68:298-307
Godwin, Ryan C; Gmeiner, William H; Salsbury Jr, Freddie R (2018) All-atom molecular dynamics comparison of disease-associated zinc fingers. J Biomol Struct Dyn 36:2581-2594
Yang, M; Forbes, M E; Bitting, R L et al. (2018) Incorporating blood-based liquid biopsy information into cancer staging: time for a TNMB system? Ann Oncol 29:311-323

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