Transgenic and genetically-engineered mutant animals provide one of the most valuable mechanisms for generating in vivo models for unraveling the molecular and genetic mechanisms of cancer and for evaluating new strategies for treatment and prevention studies. Efficient generation of transgenic animals requires great technical skill that is acquired only after extensive experience with animal husbandry and breeding, animal surgery, embryo manipulation and micromanipulation. The transgenic Animal Shared Service makes transgenic technology accessible and affordable to the members.of the University of Wisconsin Comprehensive Cancer Center. It uses the personnel, facilities and equipment of the campus-wide University of Wisconsin Biotechnology Center's Transgenic Animal Facility. Services currently provided by the Transgenic Animals Core include: Generation of transgenic mice and rats by pronuclear microinjection Cryopreservation of mouse and rat embryos ? Generation of knockout mice: generation of targeting vectors, generation of targeted embryonic stem (ES) cell clones, and generation of chimeric mice by injection of ES cells into host blastocyst Strain rederivations and ovarian transplants Other miscellaneous molecular biology and embryo services related to transgenic and knockout mouse production Consulting and training In addition, the staff are available for consulting at any point in the process of using the services. Considerable time is spent discussing experimental design and providing guidance on processes that they themselves are or will """"""""be carrying out. They also train investigators in techniques such as superovulating, mating, and collecting reproductive tracts from female mice for embryo cryopreservation, isolating DMA for pronuclear micronjections, mouse handling and breeding protocols, and cutting tails and genomic DNA preparation Southern blotting to identify correctly mutated ES cells.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014520-36
Application #
7809606
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2009-04-01
Budget End
2010-03-31
Support Year
36
Fiscal Year
2009
Total Cost
$139,288
Indirect Cost
Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715
Chute, Colleen; Yang, Xinhai; Meyer, Kristy et al. (2018) Syndecan-1 induction in lung microenvironment supports the establishment of breast tumor metastases. Breast Cancer Res 20:66
Farnoodian, Mitra; Sorenson, Christine M; Sheibani, Nader (2018) Negative Regulators of Angiogenesis, Ocular Vascular Homeostasis, and Pathogenesis and Treatment of Exudative AMD. J Ophthalmic Vis Res 13:470-486
Nayak, Amruta P; Kapur, Arvinder; Barroilhet, Lisa et al. (2018) Oxidative Phosphorylation: A Target for Novel Therapeutic Strategies Against Ovarian Cancer. Cancers (Basel) 10:
Litzelman, Kristin; Keller, Abiola O; Tevaarwerk, Amye et al. (2018) Adequacy of Depression Treatment in Spouses of Cancer Survivors: Findings From a Nationally Representative US Survey. J Gen Intern Med 33:869-876
Gurel, Zafer; Sheibani, Nader (2018) O-Linked ?-N-acetylglucosamine (O-GlcNAc) modification: a new pathway to decode pathogenesis of diabetic retinopathy. Clin Sci (Lond) 132:185-198
Li, Chao; Yu, Jiaquan; Paine, Paxton et al. (2018) Double-exclusive liquid repellency (double-ELR): an enabling technology for rare phenotype analysis. Lab Chip 18:2710-2719
Braun, Rudolf K; Chetty, Chandramu; Balasubramaniam, Vivek et al. (2018) Intraperitoneal injection of MSC-derived exosomes prevent experimental bronchopulmonary dysplasia. Biochem Biophys Res Commun 503:2653-2658
Gangnon, Ronald E; Stout, Natasha K; Alagoz, Oguzhan et al. (2018) Contribution of Breast Cancer to Overall Mortality for US Women. Med Decis Making 38:24S-31S
Turk, Anita A; Wisinski, Kari B (2018) PARP inhibitors in breast cancer: Bringing synthetic lethality to the bedside. Cancer 124:2498-2506
Liu, Ting; Shi, Changzheng; Duan, Linqi et al. (2018) A highly hemocompatible erythrocyte membrane-coated ultrasmall selenium nanosystem for simultaneous cancer radiosensitization and precise antiangiogenesis. J Mater Chem B 6:4756-4764

Showing the most recent 10 out of 1528 publications