The goal of the Proteomics Core is to provide mass spectrometry-based protein identification and protein characterization services to UNC researchers. These services include protein identification from gels, and solution, identification of co-immunoprecipitated proteins, and protein characterization, including mapping of phosphorylation sites. Recently, in response to the need of the researchers, the Core has implemented a new program in differential protein expression analysis, based on Amersham's DIGE technique. The Core is led by the faculty director, Dr. Christoph Borchers, the Core Director, Carol Parker, and the Assistant Director, Dr. Maria Warren. The Core adds value to the Center by supporting over twenty-one grant proposals for UNC LCCC members. Highlights of research supported by the Core include two 2003 Nature publications based on the work done in the Core: one Nature paper involved the identification of new member of the cullin complex which is involved in cell cycle regulation (for Dr. Y. Xiong); the other Nature publication involved the identification of a phosphorylation site which is critical for cellular mobility, which has implications for metastasis (for Dr. K. Jacobson). Future plans for the Core include expanding the differential protein expression program, adding to the current program in accurate molecular weight determination of intact proteins, and continuing method development for the determination of phosphorylation and ubiquitination sites.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee G - Education (NCI)
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University of North Carolina Chapel Hill
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Zuze, Takondwa; Painschab, Matthew S; Seguin, Ryan et al. (2018) Plasmablastic lymphoma in Malawi. Infect Agent Cancer 13:22
Wang, Jeremy R; Holt, James; McMillan, Leonard et al. (2018) FMLRC: Hybrid long read error correction using an FM-index. BMC Bioinformatics 19:50
Lee, Janie M; Abraham, Linn; Lam, Diana L et al. (2018) Cumulative Risk Distribution for Interval Invasive Second Breast Cancers After Negative Surveillance Mammography. J Clin Oncol 36:2070-2077
Shen, Hui; Shih, Juliann; Hollern, Daniel P et al. (2018) Integrated Molecular Characterization of Testicular Germ Cell Tumors. Cell Rep 23:3392-3406
Shao, Wenwei; Chen, Xiaojing; Samulski, Richard J et al. (2018) Inhibition of antigen presentation during AAV gene therapy using virus peptides. Hum Mol Genet 27:601-613
Gao, Yanzhe; Kardos, Jordan; Yang, Yang et al. (2018) The Cancer/Testes (CT) Antigen HORMAD1 promotes Homologous Recombinational DNA Repair and Radioresistance in Lung adenocarcinoma cells. Sci Rep 8:15304
Schaefer, Kristina N; Bonello, Teresa T; Zhang, Shiping et al. (2018) Supramolecular assembly of the beta-catenin destruction complex and the effect of Wnt signaling on its localization, molecular size, and activity in vivo. PLoS Genet 14:e1007339
Bonacci, Thomas; Suzuki, Aussie; Grant, Gavin D et al. (2018) Cezanne/OTUD7B is a cell cycle-regulated deubiquitinase that antagonizes the degradation of APC/C substrates. EMBO J 37:
Zhang, Zhi-Min; Lu, Rui; Wang, Pengcheng et al. (2018) Structural basis for DNMT3A-mediated de novo DNA methylation. Nature 554:387-391
Desai, Neelam V; Torous, Vanda; Parker, Joel et al. (2018) Intrinsic molecular subtypes of breast cancers categorized as HER2-positive using an alternative chromosome 17 probe assay. Breast Cancer Res 20:75

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