The Transgenic and Chimeric Mouse Facility has been continuously funded by the NCI CCSG since 1989. Drs. Stephen Liebhaber and Nancy Cooke, Professors of Genetics and Medicine, have provided leadership as Co- Directors to this Shared Resource since 1993. Both individuals are experienced investigators with considerable expertise in transgenic and chimeric mouse generation and analysis, and mammalian gene targeting. An experienced technical team led by Dr. Jean Richa provides expertise in a range of transgenic technologies, enabling the facility to regularly introduce new and improved services. Among the services provided by the Transgenic and Chimeric Mouse Facility are: generation of transgenic mice via microinjections of DNA into fertilized oocyte; generation of chimeric mice via injection of embryonic stem cells into blastocysts; direct genome editing (Zn finger, TALEN, and CRISPR/Cas9) by microinjection of RNA and DNA into fertilized embryos; embryo re-derivation; embryo and sperm cryopreservation; in vitro fertilization; centralized and monitored cryopreservation storage; and maintenance of generally useful mouse lines including those for recombination-activation gene expression. The facility uses state-of-the-art laser conditioning of the zona to facilitate IVF and has intracytoplasmic sperm injection capability on-line to complement the in vitro fertilization service. Newly developed services during the current funding period include the major expansion of cryopreservation services with corresponding expansion of the centralized and dedicated cryopreservation facility, integration of mouse ES microinjection service with a centralized gene knock-out core facility, and direct genome editing by Zn-finger, TALEN and CRISPR-Cas9 technologies. In the reporting period (10/01/13- 09/30/14), Cancer Center members accounted for 46% of total Facility users and those with peer-reviewed funding accounted for 42%. This compares favorably with our request for 21% of the total budget from the CCSG. Additional Institutional support is provided in the form of funding for equipment maintenance and facility infrastructure upgrades and maintenance.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA016520-44
Application #
9836817
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2019-12-01
Budget End
2020-11-30
Support Year
44
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Morrison, Alexander H; Byrne, Katelyn T; Vonderheide, Robert H (2018) Immunotherapy and Prevention of Pancreatic Cancer. Trends Cancer 4:418-428
Ojha, Rani; Leli, Nektaria M; Onorati, Angelique et al. (2018) ER translocation of the MAPK pathway drives therapy resistance in BRAF mutant melanoma. Cancer Discov :
Yan, Lesan; Amirshaghaghi, Ahmad; Huang, Dennis et al. (2018) Protoporphyrin IX (PpIX)-Coated Superparamagnetic Iron Oxide Nanoparticle (SPION) Nanoclusters for Magnetic Resonance Imaging and Photodynamic Therapy. Adv Funct Mater 28:
Waxman, Adam J; Clasen, Suparna; Hwang, Wei-Ting et al. (2018) Carfilzomib-Associated Cardiovascular Adverse Events: A Systematic Review and Meta-analysis. JAMA Oncol 4:e174519
Han, Joseph; Lachance, Catherine; Ricketts, M Daniel et al. (2018) The scaffolding protein JADE1 physically links the acetyltransferase subunit HBO1 with its histone H3-H4 substrate. J Biol Chem 293:4498-4509
Reshef, Ran; Ganetsky, Alex; Acosta, Edward P et al. (2018) Extended CCR5 Blockade for Graft-versus-Host Disease Prophylaxis Improves Outcomes of Reduced-Intensity Unrelated Donor Hematopoietic Cell Transplantation: A Phase II Clinical Trial. Biol Blood Marrow Transplant :
Gangadhar, Tara C; Savitch, Samantha L; Yee, Stephanie S et al. (2018) Feasibility of monitoring advanced melanoma patients using cell-free DNA from plasma. Pigment Cell Melanoma Res 31:73-81
Rosenfeld, Aaron M; Meng, Wenzhao; Luning Prak, Eline T et al. (2018) ImmuneDB, a Novel Tool for the Analysis, Storage, and Dissemination of Immune Repertoire Sequencing Data. Front Immunol 9:2107
Lang, Fengchao; Sun, Zhiguo; Pei, Yonggang et al. (2018) Shugoshin 1 is dislocated by KSHV-encoded LANA inducing aneuploidy. PLoS Pathog 14:e1007253
Buljan, Vlado A; Graeber, Manuel B; Holsinger, R M Damian et al. (2018) Calcium-axonemal microtubuli interactions underlie mechanism(s) of primary cilia morphological changes. J Biol Phys 44:53-80

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