DNA SequencingBegun in 1996 and initially funded by the Director's discretionary funds, the DNA Sequencing SharedResource provides fast, on-site sequencing services to Moores UCSD Cancer Center Members. Eighty-sixper cent of the utilization of this Shared Resource is from Cancer Center members while CCSG support forthis Resource accounts for 30% of the Resource's annual operating expenses. Support of this Resource bythe CCSG provides very cost effective DNA sequencing services to Cancer Center Members.The Resouce has developed a campus-wide reputation for providing consistently high quality, fast,long-read (1000+) and inexpensive DNA sequencing services. SNP analyses and microsatellitesizing for genome research or other applications is provided through the use of GeneScan ,Genotyper and GeneMapper software used with our two Applied Biosystems 3130x1 GeneticAnalyzers.The speedy, convenient service established by this Resource for its users is complemented by theready availability of expert consultation on how to solve difficult sequencing problems, relating toparticular runs of bases in some parts of the genome. Such advice and help is a special value-addedfeature this facility provides for Center investigators, which is not easily obtained commercially.Our services have been designed to maximize efficiency for the investigator. Investigators use our web siteto register their orders and define their template names. The Resource provides a daily courier service topick up in each lab primers and templates ready for sequencing. Sequencing data is returned electronicallywithin a turnaround time of 24 to 48 hours.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA023100-23
Application #
7515259
Study Section
Subcommittee G - Education (NCI)
Project Start
2007-06-01
Project End
2012-04-30
Budget Start
2007-06-01
Budget End
2008-04-30
Support Year
23
Fiscal Year
2007
Total Cost
$135,870
Indirect Cost
Name
University of California San Diego
Department
Type
DUNS #
804355790
City
La Jolla
State
CA
Country
United States
Zip Code
92093
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