Mouse Modeling Mice are by far the most commonly used vertebrate research model organism for biomedical research, and the ability to generate efficiently genetically-engineered mouse models (GEMM), maintain those mice, and use them for sophisticated experimentation is the basis for the dominance of mice for mammalian biomedical research. As with most biomedical research platforms, the utilization of mice is complex, and often researchers need specialized support to accomplish their mouse experiments. The Mouse Modeling (MM) Shared Resource is focused on supporting researchers doing mouse experimentation by offering a broad range of services that are adapted for specific experimental needs, updating services with the latest technological approaches and doing this service in an efficient and cost-effective manner. By keeping the focus on flexibility, cutting-edge capability and efficiency of services, we have been able to support mouse model experimentation by many researchers and remain financially solvent for over 21 years. The vast majority of the experiments we support are cancer-focused, with other major areas including neuroscience, infectious disease and antibody generation. During the current award period services were performed for 27 Cancer Center Members, 25 of whom now are Members of all 4 NCCC Programs (CPS [1], CBT [11], ICI [10], and TEC [3]). NCCC Funded Members represented 37% of Total MM projects served (549), and we are requesting only 24% of the Total MM Operating Budget from CCSG support. As an example of value added to NCCC research, ?Floxed? ID2 mice were generated by the MM Shared Resource for a study investigating the role of ID2 in glioma biology, and now these mice are available for other researchers, here and outside Dartmouth, and can be combined with Cre-expressing mice to study the biology of ID2, a gene that that plays a role in multiple different cancers. In a second study involving genetically engineered mice, including a knock-in of a Cre-expression-regulated allele of constitutively active P110? of P13 kinase, the resulting model was the first reported rapidly developing murine breast tumor model that generates predominantly adenocarcinoma breast tumors, pathologically similar to human breast cancer. In contrast, other models are either very slow to develop or generate sarcomatoid breast tumors, which are rare in human breast cancer. MM?s menu of services constantly is evolving to respond to changing technologies and changing needs of new and established faculty at Dartmouth. Recently, we have been more involved providing mouse model experimentation services to support labs that either are not experienced with mouse experimentation or do not have sufficient lab personnel to support needed mouse studies, such as engineering faculty from Dartmouth?s Thayer School of Engineering, or research clinical faculty from Dartmouth-Hitchcock. Another new area of focus for MM is the use of CRISPR/Cas9 to make directly targeted genetic modifications in mouse zygotes with no intermediate tissue culture step with ES cells. Multiple mouse models have been made, and we continue to develop skills with this powerful technology.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA023108-41
Application #
9855309
Study Section
Subcommittee I - Transistion to Independence (NCI)
Project Start
Project End
Budget Start
2019-12-01
Budget End
2020-11-30
Support Year
41
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Dartmouth College
Department
Type
DUNS #
041027822
City
Hanover
State
NH
Country
United States
Zip Code
03755
Yang, Wei; Hosford, Sarah R; Traphagen, Nicole A et al. (2018) Autophagy promotes escape from phosphatidylinositol 3-kinase inhibition in estrogen receptor-positive breast cancer. FASEB J 32:1222-1235
Landon, Bruce E; Keating, Nancy L; Onnela, Jukka-Pekka et al. (2018) Patient-Sharing Networks of Physicians and Health Care Utilization and Spending Among Medicare Beneficiaries. JAMA Intern Med 178:66-73
Ghali, Fady; Celaya, Maria; Laviolette, Michael et al. (2018) Does Travel Time to a Radiation Facility Impact Patient Decision-Making Regarding Treatment for Prostate Cancer? A Study of the New Hampshire State Cancer Registry. J Rural Health 34 Suppl 1:s84-s90
Goodwin, Eileen; Gilman, Morgan S A; Wrapp, Daniel et al. (2018) Infants Infected with Respiratory Syncytial Virus Generate Potent Neutralizing Antibodies that Lack Somatic Hypermutation. Immunity 48:339-349.e5
Buckey, Jay C; Phillips, Scott D; Anderson, Allison P et al. (2018) Microgravity-induced ocular changes are related to body weight. Am J Physiol Regul Integr Comp Physiol 315:R496-R499
He, Xiaofei; Schifferdecker, Karen E; Ozanne, Elissa M et al. (2018) How Do Women View Risk-Based Mammography Screening? A Qualitative Study. J Gen Intern Med 33:1905-1912
Marra, Kayla; LaRochelle, Ethan P; Chapman, M Shane et al. (2018) Comparison of Blue and White Lamp Light with Sunlight for Daylight-Mediated, 5-ALA Photodynamic Therapy, in vivo. Photochem Photobiol 94:1049-1057
Thompson, Jeffrey A; Christensen, Brock C; Marsit, Carmen J (2018) Pan-Cancer Analysis Reveals Differential Susceptibility of Bidirectional Gene Promoters to DNA Methylation, Somatic Mutations, and Copy Number Alterations. Int J Mol Sci 19:
Brooks, J M; Titus, A J; Bruce, M L et al. (2018) Depression and Handgrip Strength Among U.S. Adults Aged 60 Years and Older from NHANES 2011-2014. J Nutr Health Aging 22:938-943
Bazett, Mark; Costa, Amanda M; Bosiljcic, Momir et al. (2018) Harnessing innate lung anti-cancer effector functions with a novel bacterial-derived immunotherapy. Oncoimmunology 7:e1398875

Showing the most recent 10 out of 1911 publications