Flow Cytometry and Cell Separation Shared Resource: Project Summary Biology Shared Resource Group The Purdue Flow Cytometry and Cell Separation Shared Resource (FC-SR) provides advanced cell and particle analysis, and sorting capabilities to Purdue University Center for Cancer Research (PCCR) members. As an analytical tool, flow cytometry allows users to identify and quantitate cellular features, organelles, or structural components using optical measures. When combined with sorting capabilities, it is possible to identify and isolate single cells that have specific properties or express specific molecules and which can then be further examined and characterized. In cancer research, where the identification and characterization of unique, specific cells with cancer-like characteristics is critical, flow cytometry and cell sorting are extremely vital and useful to today's cancer researcher. Purdue's FC-SR provides a broad range of services that supported 24 Purdue Center for Cancer Research (PCCR) investigators' research projects from all four of PCCR's Research Programs in this current year. The FC-SR, located in the Bindley Bioscience Center (BBC) of Discovery Park, has grown considerably in both its technical expertise and instrument capabilities since its total re-organization in March 2009. A major emphasis of the facility is live cell sorting of mouse and human cells under BSL-2 conditions. In 2010 with support from an NIH S10 Shared Instrument Grant, the FC-SR obtained a high-throughput LEAP image scanning cytometer. This instrument adds an additional dimension to sorting of attached cells by laser ablation and serves as a bridge between flow cytometry and image analysis capabilities. The FC-SR also operates a FACS Aria III Sorter that serves as the primary workhorse for analysis and cell sorting. Currently, the facilty also operates a FC500 Cell Analyzer for routine cell analyses. The data analysis capabilities of the facility have advanced greatly with the addition of many FlowJo site licenses and the development of new advanced data analysis capabilities. Perhaps the most important resource provided by the FC-SR is the expertise that the staff shares. Many of FC-SR's clients have little, if any, experience conducting cytometry analysis or designing experiments using the BD FACS Aria III cell sorter. In the absence of the FC-SR and its experienced staff, these investigators would have tremendous difficulty in generating cytometry data necessary for competitive external funding.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA023168-38
Application #
9516903
Study Section
Special Emphasis Panel (ZCA1)
Project Start
Project End
Budget Start
2018-07-01
Budget End
2019-06-30
Support Year
38
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Purdue University
Department
Type
DUNS #
072051394
City
West Lafayette
State
IN
Country
United States
Zip Code
47907
Lee, Hyeon Jeong; Li, Jie; Vickman, Renee E et al. (2018) Cholesterol Esterification Inhibition Suppresses Prostate Cancer Metastasis by Impairing the Wnt/?-catenin Pathway. Mol Cancer Res 16:974-985
Bhandari, Pushpak; Novikova, Gloriia; Goergen, Craig J et al. (2018) Ultrasound beam steering of oxygen nanobubbles for enhanced bladder cancer therapy. Sci Rep 8:3112
Dhawan, Deepika; Hahn, Noah M; Ramos-Vara, José A et al. (2018) Naturally-occurring canine invasive urothelial carcinoma harbors luminal and basal transcriptional subtypes found in human muscle invasive bladder cancer. PLoS Genet 14:e1007571
Shinde, Aparna; Libring, Sarah; Alpsoy, Aktan et al. (2018) Autocrine Fibronectin Inhibits Breast Cancer Metastasis. Mol Cancer Res 16:1579-1589
Ghosh, Arun K; Ghosh, Koena; Brindisi, Margherita et al. (2018) Design, synthesis, X-ray studies, and biological evaluation of novel BACE1 inhibitors with bicyclic isoxazoline carboxamides as the P3 ligand. Bioorg Med Chem Lett 28:2605-2610
Thompson, Taylor J; Han, Bumsoo (2018) Analysis of adhesion kinetics of cancer cells on inflamed endothelium using a microfluidic platform. Biomicrofluidics 12:042215
Alpsoy, Aktan; Dykhuizen, Emily C (2018) Glioma tumor suppressor candidate region gene 1 (GLTSCR1) and its paralog GLTSCR1-like form SWI/SNF chromatin remodeling subcomplexes. J Biol Chem 293:3892-3903
Larocque, Elizabeth A; Naganna, N; Opoku-Temeng, Clement et al. (2018) Alkynylnicotinamide-Based Compounds as ABL1 Inhibitors with Potent Activities against Drug-Resistant CML Harboring ABL1(T315I) Mutant Kinase. ChemMedChem 13:1172-1180
Kumari, Rashmi; Silic, Martin R; Jones-Hall, Yava L et al. (2018) Identification of RECK as an evolutionarily conserved tumor suppressor gene for zebrafish malignant peripheral nerve sheath tumors. Oncotarget 9:23494-23504
VerHeul, Ross; Sweet, Craig; Thompson, David H (2018) Rapid and simple purification of elastin-like polypeptides directly from whole cells and cell lysates by organic solvent extraction. Biomater Sci 6:863-876

Showing the most recent 10 out of 436 publications