The Antibody Shared Resource provides a central site for the production of monoclonal and polyclonal antibodies by CSHL Cancer Center personnel. The purpose of this Shared Resource is to provide the resources and expertise to generate these valuable reagents in a highly efficient and cost-effective manner. In the ease of monoclonal antibodies, CSHL Cancer Center scientists provide specific antigens, and the Shared Resource personnel handle all stages of immunization, fusion, primary screening of hybridomas, cell culture, single-cell cloning, freezing and storage of hybridoma lines, and large-scale production of monoclonal antibodies in cell culture or in vivo. Immunization, test bleeds, and production of ascites fluids are coordinated with the personnel of the Animal Shared Resource. Specialized monoclonal antibody screens are closely coordinated with individual scientists. Polyclonal antibody production is outsourced, but the Shared Resource personnel are in charge of processing and distribution of samples, thus reducing the costs and maximizing efficiency. The Shared Resource also tests and implements new techniques, reagents, and assay improvements related to the different stages of antibody production.

Public Health Relevance

The Antibody Shared Resource provides essential services, promotes valuable scientific and technical interactions, and stimulates cancer research that is heavily dependent on high-quality antibody reagents. These reagents help advance basic knowledge about cancer, as well as therapeutics development, and in some cases they also serve to detect and quantitate useful cancer biomarkers.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA045508-24
Application #
8340283
Study Section
Subcommittee G - Education (NCI)
Project Start
2011-08-17
Project End
2016-07-31
Budget Start
2011-08-17
Budget End
2012-07-31
Support Year
24
Fiscal Year
2011
Total Cost
$201,564
Indirect Cost
Name
Cold Spring Harbor Laboratory
Department
Type
DUNS #
065968786
City
Cold Spring Harbor
State
NY
Country
United States
Zip Code
11724
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