Flow Cytometry is an important analytical and cell purification tool in cancer and immunological research. Modern cancer analysis requires innovative procedures that include both imaging methods and non-imaging analysis, such as that provided by flow cytometry. Flow cytometry allows the isolation of pure cell populations comprising less than 0.01% of a complex mixture. In addition, as an analytical tool it permits statistical analysis of cell population and cell function changes, apoptosis, intracellular protein expression, and gene transfection for example. Innovative methods that contribute to modern cancer research include gene expression detection with green fluorescent protein, parameter cytometry, apoptotic effect of chemotherapy, multi-drug resistance evaluation, and minimal residual disease detection. In addition, the integrated use of cell isolation (by flow cytometry) and downstream assessment of isolated cells (by proteomics or genomics) offers a powerful application of technologies offered by multiple Resources within the Knight Cancer Institute. High quality instrumentation is available to Knight Cancer Institute members in the form of two cell sorters and three analytical instruments. The Flow Cytometry Shared Resource provides consultation, training/teaching, technical expertise, and technical services in flow cytometry to meet the needs of Knight Cancer Institute members.

Public Health Relevance

The Flow Cytometry Shared Resource offers state of the art equipment and support to Knight Cancer Institute researchers studying a wide array of human diseases. The resource positively impacts the study of hematopoietic and non-hematopoietic cancers, infectious diseases, inflammatory diseases, autoimmune diseases, and graft versus host disease;as well as stem cell biology and regenerative medicine.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA069533-15
Application #
8381618
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2012-07-01
Budget End
2013-06-30
Support Year
15
Fiscal Year
2012
Total Cost
$46,503
Indirect Cost
$24,882
Name
Oregon Health and Science University
Department
Type
DUNS #
096997515
City
Portland
State
OR
Country
United States
Zip Code
97239
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