The mission of the Genome Engineering Shared Resource (GESR) is to provide state-of-the-art services in precision genome engineering of mammalian cell lines to Masonic Cancer Center (MCC) researchers. Specifically, the GESR is capable of genetically engineering human cell lines tailored to each principal investigator's (PI's) individual specifications. These services include, but are not limited to 1) single guide RNA (sgRNA) design; 2) sgRNA validation; 3) sgRNA expression vector construction; 4) design of CRISPR (Clustered Regularly Interspersed Palindromic Repeats)-associated 9 (Cas9) protein expression vector reagents; 5) simple, single-locus knockouts for non-essential genes; 6) construction of conditionally null alleles for essential genes; 7) construction of single-amino-acid knock-in mutations; 8) construction of single- nucleotide knock-ins; 9) construction of multi-allelic knockout cell lines; and 10) in collaboration with the Mouse Genetics Laboratory (MGL) Shared Resource, construction of RNA- and protein-based CRISPR/Cas9 reagents for the generation of transgenic mice. These services are essential to the mission of the MCC because genetic alteration of human cancer cell lines allows PIs the ability to design mechanistic and therapeutic experiments related to the characterization and investigation of specific cancers. Moreover, although the demand for genetically modified human cell lines is high, the steps involved in the process of genome editing are still technically challenging and time consuming and demand resources and a level of expertise not found in most clinical laboratories. The GESR is capable of providing these services at a very cost-effective price (well below most commercial sources) and can usually generate the requisite reagent or cell line as quickly (or more quickly) than most commercial enterprises. The GESR is co-led by Drs. Eric A. Hendrickson and Branden S. Moriarity and coordinated by Brian Ruis with support from 2 laboratory personnel. The GESR has been in operation since October 2015.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA077598-22
Application #
9859361
Study Section
Subcommittee H - Clinical Groups (NCI)
Project Start
Project End
Budget Start
2020-02-01
Budget End
2021-01-31
Support Year
22
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of Minnesota Twin Cities
Department
Type
DUNS #
555917996
City
Minneapolis
State
MN
Country
United States
Zip Code
55455
Ustun, C; Brunstein, C; DeFor, T et al. (2018) Importance of conditioning regimen intensity, MRD positivity, and KIR ligand mismatch in UCB transplantation. Bone Marrow Transplant 53:97-100
How, Joan; Vij, Kiran R; Ebadi, Maryam et al. (2018) Prognostic value of prior consolidation in acute myeloid leukemia patients undergoing hematopoietic cell transplantation in minimal residual disease-negative first complete remission. Am J Hematol 93:E381-E383
Kaizer, Alexander M; Hobbs, Brian P; Koopmeiners, Joseph S (2018) A multi-source adaptive platform design for testing sequential combinatorial therapeutic strategies. Biometrics 74:1082-1094
Williams, Shelly M; Sumstad, Darin; Kadidlo, Diane et al. (2018) Clinical-scale production of cGMP compliant CD3/CD19 cell-depleted NK cells in the evolution of NK cell immunotherapy at a single institution. Transfusion 58:1458-1467
Li, Danni; Huang, Fangying; Zhao, Yingchun et al. (2018) Plasma lipoproteome in Alzheimer's disease: a proof-of-concept study. Clin Proteomics 15:31
Sperduto, Paul W; Deegan, Brian J; Li, Jing et al. (2018) Estimating survival for renal cell carcinoma patients with brain metastases: an update of the Renal Graded Prognostic Assessment tool. Neuro Oncol 20:1652-1660
Yun, Byeong Hwa; Bellamri, Medjda; Rosenquist, Thomas A et al. (2018) Method for Biomonitoring DNA Adducts in Exfoliated Urinary Cells by Mass Spectrometry. Anal Chem 90:9943-9950
Hwa Yun, Byeong; Guo, Jingshu; Bellamri, Medjda et al. (2018) DNA adducts: Formation, biological effects, and new biospecimens for mass spectrometric measurements in humans. Mass Spectrom Rev :
Owen, David L; Mahmud, Shawn A; Vang, Kieng B et al. (2018) Identification of Cellular Sources of IL-2 Needed for Regulatory T Cell Development and Homeostasis. J Immunol 200:3926-3933
Rashidi, Armin; Shanley, Ryan; Yohe, Sophia L et al. (2018) Association between recipient TNF rs361525 and acute GVHD: results from analysis of BMT CTN-0201 samples. Bone Marrow Transplant 53:1069-1071

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