There is increasing evidence that oxidative damage and free radicals, with sequelae of altered cellular energetics and protein alterations, are important in the initiation, promotion, invasion, metastasis, and treatment of cancers. Moreover, oxidative damage and free radicals also are implicated in the side effects of normal tissue injury following cancer therapy, including chemotherapy, radiafion, and biological modifiers. The Free Radical Biology in Cancer Shared Resource Facility (FRBC SRF) was established to provide expertise in and analyses of free radicals, cellular energetics, and reactive species, as well as proteomics in cancer and cancer biology for Markey Cancer Center (MCC) investigators who perform basic, pre-clinical, and clinical research. The four basic services provided by the FRBC SRF are: 1) Analysis of markers of oxidative and nitrosative stress;2) Molecular biological manipulation of biological systems with which to investigate redox signals, including measurements of mitochondrial function by Seahorse technology;3) Proteomics identification of differentially expressed, differentially oxidized, or differentially covalently modified proteins in various cancer-related systems;4) Electron paramagnetic resonance (EPR) detection of free radicals. Development of new applications and methodology for better understanding of the roles of free radicals in cancer and cancer chemotherapy and technical assistance with grant and manuscript preparation by MCC investigators will also be provided. Included in these functions of the considerable expertise of the FRBC SRF Technical Advisory Committee to educate MCC investigators on proper sample handling and preparation methods so that reliable, precise, and artifact-free assay results are obtained. Multiple investigators from all four research programs of the MCC (Redox Injury and Repair [RR];Cancer Cell Biology and Signaling [CS];Cancer Prevention and Control [CP];and Drug Discovery, Delivery and Translational Therapeutics [DT]) have used one or more services of the FRBC SRF. Indeed, even as a relatively new shared resource, the FRBC SRF still had usage by 26% of MCC investigators.

Public Health Relevance

Damaging free radicals are produced by cancers and with various cancer treatments. When cells are damaged, evidence of free radical-induced oxidative or nitrosative stress becomes apparent. The Free Radical Biology in Cancer Shared Resource Facility addresses these issues for members of the MCC by determining markers of oxidative stress, measuring oxidative stress-mediated mitochondrial dysfunction using Seahorse technology, and identifying aberrant cellular proteins using proteomics.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
Project #
Application #
Study Section
Subcommittee B - Comprehensiveness (NCI)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Kentucky
United States
Zip Code
Rychahou, Piotr; Bae, Younsoo; Reichel, Derek et al. (2018) Colorectal cancer lung metastasis treatment with polymer-drug nanoparticles. J Control Release 275:85-91
Kosmac, Kate; Peck, Bailey D; Walton, R Grace et al. (2018) Immunohistochemical Identification of Human Skeletal Muscle Macrophages. Bio Protoc 8:
Frohman, Heather A; Rychahou, Piotr G; Li, Jing et al. (2018) Development of murine bariatric surgery models: lessons learned. J Surg Res 229:302-310
Chaiswing, Luksana; St Clair, William H; St Clair, Daret K (2018) Redox Paradox: A Novel Approach to Therapeutics-Resistant Cancer. Antioxid Redox Signal 29:1237-1272
Chauhan, Aman; Farooqui, Zainab; Murray, Le Aundra et al. (2018) Capecitabine and Temozolomide in Neuroendocrine Tumor of Unknown Primary. J Oncol 2018:3519247
Zhang, Xiaofei; Zhang, Yi; Han, Erik Y et al. (2018) Classification of Whole Mammogram and Tomosynthesis Images Using Deep Convolutional Neural Networks. IEEE Trans Nanobioscience 17:237-242
Li, Jing; Song, Jun; Li, Xian et al. (2018) FFAR4 Is Involved in Regulation of Neurotensin Release From Neuroendocrine Cells and Male C57BL/6 Mice. Endocrinology 159:2939-2952
Rodriguez, Sharon D; Vanderford, Nathan L; Huang, Bin et al. (2018) A Social-Ecological Review of Cancer Disparities in Kentucky. South Med J 111:213-219
Chauhan, Aman; Yu, Qian; Ray, Neha et al. (2018) Global burden of neuroendocrine tumors and changing incidence in Kentucky. Oncotarget 9:19245-19254
Huang, Bin; Pollock, Elizabeth; Zhu, Li et al. (2018) Ranking composite Cancer Burden Indices for geographic regions: point and interval estimates. Cancer Causes Control 29:279-287

Showing the most recent 10 out of 359 publications