The Islet Cell Biology Core supports the overall mission of the Penn DRC to prevent, treat, and cure diabetes. Reduced islet beta-cell numbers and function underlie the progression of the full spectrum of diabetes. It is therefore essential for all laboratories investigating causes and potential cures for diabetes to be able to study islet function in relation to their specific models and molecules of interest. The objective of the Islet Cell Biology Core is to provide DRC members with state of the art support including experiment design, islet isolation, and performance of and/or training in an expansive range of assays for physiological and morphological assessment of pancreatic islet function and growth. The Core offers a range of services that generally begins with Islet isolation from rodent models and may be followed by a period of culture by the core. Islet hormone secretion can be assessed in static """"""""batch"""""""" incubations or by more informative perifusions that require larger numbers of islets and expensive immunoassays. Depending on the needs and capacity of the investigator laboratory, the core may provide these services in an ongoing manner or it may provide critical training to allow the investigator laboratory to perform the experiments independently over time. The core also has the advanced technology and expertise to perform islet and cell fluorescence imaging (Ca{i}[2+]), perifusion with respirometry, and """"""""closed"""""""" respirometry experiments for our investigators. A major advance of the previous grant cycle was an enhanced focus on human islet physiology, capitalizing on the unique strengths of the Penn DRC environment in the area of human islet procurement. By providing unique services and expertise, and continually developing state-of-the art analysis of islet structure and function, the Islet Cell Biology Core remains a critical and valuable component of the Penn DRC.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK019525-38
Application #
8638930
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2014-04-01
Budget End
2015-03-31
Support Year
38
Fiscal Year
2014
Total Cost
$187,419
Indirect Cost
$71,367
Name
University of Pennsylvania
Department
Type
DUNS #
042250712
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Kim, Yong Hoon; Marhon, Sajid A; Zhang, Yuxiang et al. (2018) Rev-erb? dynamically modulates chromatin looping to control circadian gene transcription. Science 359:1274-1277
Mowel, Walter K; Kotzin, Jonathan J; McCright, Sam J et al. (2018) Control of Immune Cell Homeostasis and Function by lncRNAs. Trends Immunol 39:55-69
Rickels, Michael R; Peleckis, Amy J; Dalton-Bakes, Cornelia et al. (2018) Continuous Glucose Monitoring for Hypoglycemia Avoidance and Glucose Counterregulation in Long-Standing Type 1 Diabetes. J Clin Endocrinol Metab 103:105-114
Guan, Dongyin; Xiong, Ying; Borck, Patricia C et al. (2018) Diet-Induced Circadian Enhancer Remodeling Synchronizes Opposing Hepatic Lipid Metabolic Processes. Cell 174:831-842.e12
Jang, Cholsoon; Chen, Li; Rabinowitz, Joshua D (2018) Metabolomics and Isotope Tracing. Cell 173:822-837
Shoshkes-Carmel, Michal; Wang, Yue J; Wangensteen, Kirk J et al. (2018) Subepithelial telocytes are an important source of Wnts that supports intestinal crypts. Nature 557:242-246
Ibrahim, Fadia; Maragkakis, Manolis; Alexiou, Panagiotis et al. (2018) Ribothrypsis, a novel process of canonical mRNA decay, mediates ribosome-phased mRNA endonucleolysis. Nat Struct Mol Biol 25:302-310
Condon, David E; Tran, Phu V; Lien, Yu-Chin et al. (2018) Defiant: (DMRs: easy, fast, identification and ANnoTation) identifies differentially Methylated regions from iron-deficient rat hippocampus. BMC Bioinformatics 19:31
Cooney, Laura G; Milman, Lauren W; Hantsoo, Liisa et al. (2018) Cognitive-behavioral therapy improves weight loss and quality of life in women with polycystic ovary syndrome: a pilot randomized clinical trial. Fertil Steril 110:161-171.e1
Williams, Bianca; Correnti, Jason; Oranu, Amanke et al. (2018) A novel role for ceramide synthase 6 in mouse and human alcoholic steatosis. FASEB J 32:130-142

Showing the most recent 10 out of 720 publications