The long-term goal of the DRC Immunoassay Core is to improve human health by supporting clinical laboratory testing services for research in diabetes mellitus. Since the last competitive review, Washington University School of Medicine has consolidated clinical research testing into the Core Laboratory for Clinical Studies (CLCS), and the DRC Immunoassay Core is fully-integrated into CLCS. This administrative change substantially improves the operational efficiency ofthe DRC Immunoassay Core and provides DRC investigators access to more automated instruments, better-trained personnel and improved quality control procedures. Importantly, institutional support for clinical research testing is focused through CLCS, and the DRC Immunoassay Core leverages this substantial support. CLCS provides expert consultation to investigators so that the most appropriate tests can be chosen while taking cost and number of samples into consideration. DRC investigators are not limited to a fixed menu of tests. Classic metabolic analytes such as insulin and glucagon as well as custom assays such as adiponectin or TNF-a are subsidized. Many other analytes are determined in CLCS at cost. CLCS has a contract with Quest Diagnostics at substantial cost savings for analytes not done at CLCS. Development of new research tests is an important goal. CLCS is currently investigating mouse insulin and plasma VEGF-a (vascular endothelial growth factor alpha) using digital single molecule counting, a promising new technique for materially improving ELISA sensitivity. CLCS is also active in validation of common laboratory analytes for FDA review under industry-sponsored contracts. Developmental research provides equipment and a level of skill that helps investigators both directly and indirectly.

Public Health Relevance

The DRC Immunoassay Core is designed to perform clinical laboratory testing to support Washington University and external research in the field of diabetes mellitus. The primary goals of the Core are to reduce substantially the high costs of specialty testing and to provide outstanding quality control so that reliable diabetes-related tests are widely available to investigators.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK020579-37
Application #
8625737
Study Section
Special Emphasis Panel (ZDK1-GRB-S)
Project Start
Project End
Budget Start
2013-12-01
Budget End
2014-11-30
Support Year
37
Fiscal Year
2014
Total Cost
$205,202
Indirect Cost
$70,201
Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Rusconi, B; Jiang, X; Sidhu, R et al. (2018) Gut Sphingolipid Composition as a Prelude to Necrotizing Enterocolitis. Sci Rep 8:10984
Chen, Yana; McCommis, Kyle S; Ferguson, Daniel et al. (2018) Inhibition of the Mitochondrial Pyruvate Carrier by Tolylfluanid. Endocrinology 159:609-621
Zhang, Yan; Rohatgi, Nidhi; Veis, Deborah J et al. (2018) PGC1? Organizes the Osteoclast Cytoskeleton by Mitochondrial Biogenesis and Activation. J Bone Miner Res 33:1114-1125
Xu, Wei; Mukherjee, Sumit; Ning, Yu et al. (2018) Cyclopropane fatty acid synthesis affects cell shape and acid resistance in Leishmania mexicana. Int J Parasitol 48:245-256
Zhang, Xiangyu; Evans, Trent D; Jeong, Se-Jin et al. (2018) Classical and alternative roles for autophagy in lipid metabolism. Curr Opin Lipidol 29:203-211
Hughes, Jing W; Bao, Yicheng K; Salam, Maamoun et al. (2018) Late-Onset T1DM and Older Age Predict Risk of Additional Autoimmune Disease. Diabetes Care :
Ban, Norimitsu; Lee, Tae Jun; Sene, Abdoulaye et al. (2018) Impaired monocyte cholesterol clearance initiates age-related retinal degeneration and vision loss. JCI Insight 3:
Ban, Norimitsu; Lee, Tae Jun; Sene, Abdoulaye et al. (2018) Disrupted cholesterol metabolism promotes age-related photoreceptor neurodegeneration. J Lipid Res 59:1414-1423
Weber, Kassandra J; Sauer, Madeline; He, Li et al. (2018) PPAR? Deficiency Suppresses the Release of IL-1? and IL-1? in Macrophages via a Type 1 IFN-Dependent Mechanism. J Immunol 201:2054-2069
Mayer, Allyson L; Zhang, Yiming; Feng, Emily H et al. (2018) Enhanced Hepatic PPAR? Activity Links GLUT8 Deficiency to Augmented Peripheral Fasting Responses in Male Mice. Endocrinology 159:2110-2126

Showing the most recent 10 out of 654 publications