The mission of the Microscopy and Histopathology Core B is to provide access to basic and cutting-edge optical and electron microscopy equipment, expertise and methods for microscopy techniques, training, service, and histology/histopathology support for innovative and cross-disciplinary GI and liver research. Core B, which is located at the Beth Israel Deaconess Medical Center (site 1) and Boston Children?s Hospital (site 2) has the following Specific Aims: 1) to provide access to resources, technologies and services unavailable in members' labs, or inaccessible in other institutional cores; 2) to extend the resources of HDDC investigators by subsidizing the costs of services, materials and equipment use; 3) to enhance the technical capabilities of established and young HDDC investigators through training in advanced technologies; and 4) to research, develop and acquire new and emerging technologies that will accelerate digestive diseases research now and in the future. In the past 5-year funding cycle, seven services were offered for Core B. Sixteen or more HDDC members used one or more of the Core B services in every quarter as measured in billable hours of service. HDDC support significantly extended grant dollars by reducing the cost of services by 25% or more for HDDC members and more than 80 papers primarily benefited from core B use. New innovations and equipment updates in the past funding cycle benefited, in particular, the electron microscopy and confocal microscopy services and their users. In the next funding cycle, two new services are proposed for core B including advanced microscopy instrumentation and animal histopathology support. The electron microscopy service intends to add capacity for high pressure freezing/freeze substitution and the confocal service will add new instrumentation and the capacity for ratio imaging. Overall, fifty eight HDDC Members anticipate use of Core B in next funding cycle. Discounted services for HDDC members will continue to extend grant dollars and priority access for HDDC members to core services should facilitate greater productivity.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK034854-35
Application #
9850254
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2019-12-01
Budget End
2020-11-30
Support Year
35
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Boston Children's Hospital
Department
Type
DUNS #
076593722
City
Boston
State
MA
Country
United States
Zip Code
02115
Kasendra, Magdalena; Tovaglieri, Alessio; Sontheimer-Phelps, Alexandra et al. (2018) Development of a primary human Small Intestine-on-a-Chip using biopsy-derived organoids. Sci Rep 8:2871
Wilen, Craig B; Lee, Sanghyun; Hsieh, Leon L et al. (2018) Tropism for tuft cells determines immune promotion of norovirus pathogenesis. Science 360:204-208
Rodriguez-Fraticelli, Alejo E; Wolock, Samuel L; Weinreb, Caleb S et al. (2018) Clonal analysis of lineage fate in native haematopoiesis. Nature 553:212-216
Franz, Kate M; Neidermyer, William J; Tan, Yee-Joo et al. (2018) STING-dependent translation inhibition restricts RNA virus replication. Proc Natl Acad Sci U S A 115:E2058-E2067
Brown, Jonathan D; Feldman, Zachary B; Doherty, Sean P et al. (2018) BET bromodomain proteins regulate enhancer function during adipogenesis. Proc Natl Acad Sci U S A 115:2144-2149
Dumontet, Typhanie; Sahut-Barnola, Isabelle; Septier, Amandine et al. (2018) PKA signaling drives reticularis differentiation and sexually dimorphic adrenal cortex renewal. JCI Insight 3:
Jirapinyo, Pichamol; Thompson, Andrew C; Kr├Âner, Paul T et al. (2018) Metabolic Effect of Foregut Exclusion Demonstrated by the Impact of Gastrogastric Fistula on Recurrence of Diabetes. J Am Coll Surg 226:259-266.e1
Syed, Ismail; Lee, Jennifer; Moraes-Vieira, Pedro M et al. (2018) Palmitic Acid Hydroxystearic Acids Activate GPR40, Which Is Involved in Their Beneficial Effects on Glucose Homeostasis. Cell Metab 27:419-427.e4
Schulman, Allison R; Kumar, Nitin; Thompson, Christopher C (2018) Transoral outlet reduction: a comparison of purse-string with interrupted stitch technique. Gastrointest Endosc 87:1222-1228
Khandekar, Melin J; Banks, Alexander S; Laznik-Bogoslavski, Dina et al. (2018) Noncanonical agonist PPAR? ligands modulate the response to DNA damage and sensitize cancer cells to cytotoxic chemotherapy. Proc Natl Acad Sci U S A 115:561-566

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