ADIPOCYTE BIOLOGY and NUTRIENT METABOLISM CORE The purposes of the Adipose Tissue Biology and Nutrient Metabolism Core are to: 1. Facilitate and foster research on mechanisms regulating white, beige and brown adipose tissue mass, distribution, nutrient signaling and depot-specific metabolic and endocrine functions in health and disease. 2. Provide easy and cost-effective access to carefully quality controlled rodent and human preadipocytes and adipocytes, and banked cell and tissues and their products (e.g. RNA, cDNA, tissue or cell lysates, conditioned media). 3. Develop and standardize methods in adipocyte and islet biology as needed by our research base in this rapidly evolving field. 4. Provide consultative advice, support and training for new investigators and established investigators from the nutrition and obesity fields. 5. Provide consultative advice, support and training in the preparation and quality assessment of metabolic tissues including hepatocytes, islets and human fibroblasts. 6. Facilitate and foster the translation of basic research findings from cell and animal models into clinical/translational studies. 7. Organize the Adipose and Metabolic Tissue Seminar Series, which serves as an incubator for new collaborations, and as a training ground for pre-doctoral students and post-doctoral fellows. 8. Organize workshops on controversial topics and the application of new technologies to our field. Services Provided By The Core 1. Adipose tissue procurement and processing 2. Isolation of stromal vascular cells and preparation of adipose stem cells (ASCs) 3. Differentiation of ASCs/preadipocytes into adipocytes: 4. Hormone and cytokine assays. Secretion of adipokines from tissue fragments or cells 5. Dissection and harvest of rodent adipose tissues. 6. Standardized protocols and analytical techniques and advice on optimal methods for metabolic studies (e.g. metabolites, lipolysis, glucose uptake and metabolism, lipogenesis, hormone signaling) Planned New ABM Core Services: ? Cell respiration and bioenergetics with the Seahorse Oxygen Analyzer. ? Mitochondria isolation from rodent liver and fat cells ? Islet isolation from rats or mice by collagenase digestion

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
2P30DK046200-26
Application #
9390336
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2017-07-01
Budget End
2018-06-30
Support Year
26
Fiscal Year
2018
Total Cost
Indirect Cost
Name
Boston Medical Center
Department
Type
DUNS #
005492160
City
Boston
State
MA
Country
United States
Zip Code
Marron, Elena M; Viejo-Sobera, Raquel; Cuatrecasas, Guillem et al. (2018) Prefronto-cerebellar neuromodulation affects appetite in obesity. Int J Obes (Lond) :
Dong, Kimberly R; Must, Aviva; Tang, Alice M et al. (2018) Food Insecurity, Morbidities, and Substance Use in Adults on Probation in Rhode Island. J Urban Health 95:564-575
Chiu, Yu-Han; Bertrand, Kimberly A; Zhang, Shumin et al. (2018) A prospective analysis of circulating saturated and monounsaturated fatty acids and risk of non-Hodgkin lymphoma. Int J Cancer 143:1914-1922
Yamamoto, Ayae; Harris, Holly R; Vitonis, Allison F et al. (2018) A prospective cohort study of meat and fish consumption and endometriosis risk. Am J Obstet Gynecol 219:178.e1-178.e10
Todd, William D; Fenselau, Henning; Wang, Joshua L et al. (2018) A hypothalamic circuit for the circadian control of aggression. Nat Neurosci 21:717-724
Perng, Wei; Rifas-Shiman, Sheryl L; Hivert, Marie-France et al. (2018) Branched Chain Amino Acids, Androgen Hormones, and Metabolic Risk Across Early Adolescence: A Prospective Study in Project Viva. Obesity (Silver Spring) 26:916-926
Kim, Hanseul; Keum, NaNa; Giovannucci, Edward L et al. (2018) Garlic intake and gastric cancer risk: Results from two large prospective US cohort studies. Int J Cancer 143:1047-1053
Heianza, Yoriko; Sun, Dianjianyi; Smith, Steven R et al. (2018) Changes in Gut Microbiota-Related Metabolites and Long-term Successful Weight Loss in Response to Weight-Loss Diets: The POUNDS Lost Trial. Diabetes Care 41:413-419
Chistol, Liem T; Bandini, Linda G; Must, Aviva et al. (2018) Sensory Sensitivity and Food Selectivity in Children with Autism Spectrum Disorder. J Autism Dev Disord 48:583-591
Thomas, Dylan D; Istfan, Nawfal W; Bistrian, Bruce R et al. (2018) Protein sparing therapies in acute illness and obesity: a review of George Blackburn's contributions to nutrition science. Metabolism 79:83-96

Showing the most recent 10 out of 876 publications