ADIPOCYTE BIOLOGY and NUTRIENT METABOLISM CORE The purposes of the Adipose Tissue Biology and Nutrient Metabolism Core are to: 1. Facilitate and foster research on mechanisms regulating white, beige and brown adipose tissue mass, distribution, nutrient signaling and depot-specific metabolic and endocrine functions in health and disease. 2. Provide easy and cost-effective access to carefully quality controlled rodent and human preadipocytes and adipocytes, and banked cell and tissues and their products (e.g. RNA, cDNA, tissue or cell lysates, conditioned media). 3. Develop and standardize methods in adipocyte and islet biology as needed by our research base in this rapidly evolving field. 4. Provide consultative advice, support and training for new investigators and established investigators from the nutrition and obesity fields. 5. Provide consultative advice, support and training in the preparation and quality assessment of metabolic tissues including hepatocytes, islets and human fibroblasts. 6. Facilitate and foster the translation of basic research findings from cell and animal models into clinical/translational studies. 7. Organize the Adipose and Metabolic Tissue Seminar Series, which serves as an incubator for new collaborations, and as a training ground for pre-doctoral students and post-doctoral fellows. 8. Organize workshops on controversial topics and the application of new technologies to our field. Services Provided By The Core 1. Adipose tissue procurement and processing 2. Isolation of stromal vascular cells and preparation of adipose stem cells (ASCs) 3. Differentiation of ASCs/preadipocytes into adipocytes: 4. Hormone and cytokine assays. Secretion of adipokines from tissue fragments or cells 5. Dissection and harvest of rodent adipose tissues. 6. Standardized protocols and analytical techniques and advice on optimal methods for metabolic studies (e.g. metabolites, lipolysis, glucose uptake and metabolism, lipogenesis, hormone signaling) Planned New ABM Core Services: ? Cell respiration and bioenergetics with the Seahorse Oxygen Analyzer. ? Mitochondria isolation from rodent liver and fat cells ? Islet isolation from rats or mice by collagenase digestion

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK046200-27
Application #
9699470
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
2019-06-01
Budget End
2020-05-31
Support Year
27
Fiscal Year
2019
Total Cost
Indirect Cost
Name
Boston Medical Center
Department
Type
DUNS #
005492160
City
Boston
State
MA
Country
United States
Zip Code
02118
Kong, Xingxing; Yao, Ting; Zhou, Peng et al. (2018) Brown Adipose Tissue Controls Skeletal Muscle Function via the Secretion of Myostatin. Cell Metab 28:631-643.e3
Nisa, Hoirun; Qi, Kevin H T; Leng, Junhong et al. (2018) The Circadian Rhythm-Related MTNR1B Genotype, Gestational Weight Gain, and Postpartum Glycemic Changes. J Clin Endocrinol Metab 103:2284-2290
Chiu, Yu-Han; Williams, Paige L; Gillman, Matthew W et al. (2018) Association Between Pesticide Residue Intake From Consumption of Fruits and Vegetables and Pregnancy Outcomes Among Women Undergoing Infertility Treatment With Assisted Reproductive Technology. JAMA Intern Med 178:17-26
Sun, Dianjianyi; Li, Xiang; Heianza, Yoriko et al. (2018) History of Asthma From Childhood and Arterial Stiffness in Asymptomatic Young Adults: The Bogalusa Heart Study. Hypertension 71:928-936
Ross, Rachel A; Leon, Silvia; Madara, Joseph C et al. (2018) PACAP neurons in the ventral premammillary nucleus regulate reproductive function in the female mouse. Elife 7:
Fadelu, Temidayo; Zhang, Sui; Niedzwiecki, Donna et al. (2018) Nut Consumption and Survival in Patients With Stage III Colon Cancer: Results From CALGB 89803 (Alliance). J Clin Oncol 36:1112-1120
Chiu, Yu-Han; Williams, Paige L; Mínguez-Alarcón, Lidia et al. (2018) Comparison of questionnaire-based estimation of pesticide residue intake from fruits and vegetables with urinary concentrations of pesticide biomarkers. J Expo Sci Environ Epidemiol 28:31-39
Ramesh, Rohan N; Burgess, Christian R; Sugden, Arthur U et al. (2018) Intermingled Ensembles in Visual Association Cortex Encode Stimulus Identity or Predicted Outcome. Neuron 100:900-915.e9
Keum, NaNa; Ha, Kyoung Hwa; Bao, Ying et al. (2018) Long-term patterns of fasting blood glucose levels and pancreatic cancer incidence. Cancer Causes Control 29:135-142
Dong, Kimberly R; Tang, Alice M; Stopka, Thomas J et al. (2018) Food acquisition methods and correlates of food insecurity in adults on probation in Rhode Island. PLoS One 13:e0198598

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