The Boston-Cambridge area is home to a large research community of diverse scientists studying Type I and Type II diabetes. At present, many investigators are attempting to carry out techniques in their own laboratories for research goals that in many cases are no longer their primary area of specialty. This is particularly true for flow cytometry and complex separations of lymphocytes with the need for tight controls on the quality of the preparations. Importantly, few flow cytometry cores are dedicated to investigators involved in diabetes research. The purpose of this Immunology Core of the Boston Area Diabetes Endocrinology Research Center (BADERC) is to financially improve and scientifically expedite the translation of excellent ongoing diabetes research through a cost efficient mechanism. This has been successfully accomplished by the Immunology Core, which has been highly utilized over the past 5 years. The Immunology Core has provided strong education to investigators and a diversity of state-of-the-art flow cytometric techniques and new robotic methods for lymphocyte separations. Remarkably, at the entire Massachusetts General Hospital, Harvard Medical School as well as Boston University no flow cytometry core for diabetes users and collaborators and for their applications central to Type 1 and Type II diabetes research existed prior to the BADERC. The Immunology Flow Cytometry Core has provided these fundamental services to the Boston-Cambridge diabetes based research community including: .Flow cytometer access, data preparation and interpretations .Flow cytometry training, education and experiment design assistance. .Flow cytometry sample analysis with sorting, multi-color detection, fluorchrome labeling, antibody purifications, DNA and Ca flux experiments, etc. .Manual and robotic human blood lymphocyte separations for standardizations of assays using robotics. These Center services have been very cost effective as well as to provide rigorous training in Immunology.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1-GRB-2)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Massachusetts General Hospital
United States
Zip Code
Kolar, Matthew J; Nelson, Andrew T; Chang, Tina et al. (2018) Faster Protocol for Endogenous Fatty Acid Esters of Hydroxy Fatty Acid (FAHFA) Measurements. Anal Chem 90:5358-5365
Todd, William D; Fenselau, Henning; Wang, Joshua L et al. (2018) A hypothalamic circuit for the circadian control of aggression. Nat Neurosci 21:717-724
Cox, Kimberly H; Oliveira, Luciana M B; Plummer, Lacey et al. (2018) Modeling mutant/wild-type interactions to ascertain pathogenicity of PROKR2 missense variants in patients with isolated GnRH deficiency. Hum Mol Genet 27:338-350
Aguayo-Mazzucato, Cristina; Bonner-Weir, Susan (2018) Pancreatic ? Cell Regeneration as a Possible Therapy for Diabetes. Cell Metab 27:57-67
McKeown, Nicola M; Dashti, Hassan S; Ma, Jiantao et al. (2018) Sugar-sweetened beverage intake associations with fasting glucose and insulin concentrations are not modified by selected genetic variants in a ChREBP-FGF21 pathway: a meta-analysis. Diabetologia 61:317-330
Vandoorne, Katrien; Rohde, David; Kim, Hye-Yeong et al. (2018) Imaging the Vascular Bone Marrow Niche During Inflammatory Stress. Circ Res 123:415-427
Ross, Rachel A; Leon, Silvia; Madara, Joseph C et al. (2018) PACAP neurons in the ventral premammillary nucleus regulate reproductive function in the female mouse. Elife 7:
Berkowitz, Seth A; Karter, Andrew J; Corbie-Smith, Giselle et al. (2018) Food Insecurity, Food ""Deserts,"" and Glycemic Control in Patients With Diabetes: A Longitudinal Analysis. Diabetes Care 41:1188-1195
Syed, Ismail; Lee, Jennifer; Moraes-Vieira, Pedro M et al. (2018) Palmitic Acid Hydroxystearic Acids Activate GPR40, Which Is Involved in Their Beneficial Effects on Glucose Homeostasis. Cell Metab 27:419-427.e4
Vujic, Ana; Lerchenm├╝ller, Carolin; Wu, Ting-Di et al. (2018) Exercise induces new cardiomyocyte generation in the adult mammalian heart. Nat Commun 9:1659

Showing the most recent 10 out of 389 publications