The primary objectives of the Biomolecular Research Core of the Diabetes and Endocrinology Research Center (DERC) are to provide high quality, cost-effective, sophisticated analytical and synthetic services, instrumentation and consultation in the areas of protein, and DNA sciences. To achieve these goals the following specific services are offered: Protein Sciences - Amino acid analysis; Edman and mass spectrometric peptide sequencing and phosphopeptide sequencing; Mass spectrometric protein and peptide analysis and determination of post-translational protein modifications; CD spectropoladmetry; Dynamic light scattering; Large and small-scale peptide synthesis and anti-peptide antigen production. Proteomic analyses using 2D PAGE and orthogonai chromatography coupled with nanospray i/ms/ms. DNA Sciences - Automated DNA sequencing, genotyping and satellite mapping; DNA chemical manipulation. Affymetrix GeneChip and custom microarray gene profiling. Quantitative RT-PCR. High throughput oligonucleotide synthesis and """"""""boutique"""""""" oligonucleotide synthesis. Expert consultation on the planning and implementation of research projects and individual experiments. These services are provided using either core personnel or the use of core instrumentation by trained investigators and students. Particular emphasis in the core is directed at the development of technologies and protocols which are in demand by investigators in the DERC. Recent expansion in the core includes a 2D gel electrophoresis service for sample preparation for mass spectrometric sequencing of proteins (proteomics), new mass spectrometers for ultra-high sensitivity protein sequence analysis, high throughput capillary DNA sequencer, high throughput oligonucleotide synthesizer; and a quantitative RT-PCR instrument for gene chip target validation and real-time PCR.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK063609-04
Application #
7550814
Study Section
Special Emphasis Panel (ZDK1)
Project Start
2006-02-01
Project End
2008-01-31
Budget Start
2006-02-01
Budget End
2007-01-31
Support Year
4
Fiscal Year
2006
Total Cost
$113,445
Indirect Cost
Name
University of Virginia
Department
Type
DUNS #
065391526
City
Charlottesville
State
VA
Country
United States
Zip Code
22904
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Cutchins, Alexis; Harmon, Daniel B; Kirby, Jennifer L et al. (2012) Inhibitor of differentiation-3 mediates high fat diet-induced visceral fat expansion. Arterioscler Thromb Vasc Biol 32:317-24
Corbin, Kathryn L; Hall, Thomas E; Haile, Ruth et al. (2011) A novel fluorescence imaging approach for comparative measurements of pancreatic islet function in vitro. Islets 3:14-20
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Johnson, Michael L; Veldhuis, Paula P; Grimmichova, Tereza et al. (2010) Validation of a deconvolution procedure (AutoDecon) for identification and characterization of fasting insulin secretory bursts. J Diabetes Sci Technol 4:1205-13
Crim, William S; Wu, Runpei; Carter, Jeffrey D et al. (2010) AGI-1067, a novel antioxidant and anti-inflammatory agent, enhances insulin release and protects mouse islets. Mol Cell Endocrinol 323:246-55
Dula, Stacey B; Jecmenica, Mladen; Wu, Runpei et al. (2010) Evidence that low-grade systemic inflammation can induce islet dysfunction as measured by impaired calcium handling. Cell Calcium 48:133-42
Cole, Banumathi K; Keller, Susanna R; Wu, Runpei et al. (2010) Valsartan protects pancreatic islets and adipose tissue from the inflammatory and metabolic consequences of a high-fat diet in mice. Hypertension 55:715-21
Kim, Hyun Bae; Kumar, Anil; Wang, Lifu et al. (2010) Lipin 1 represses NFATc4 transcriptional activity in adipocytes to inhibit secretion of inflammatory factors. Mol Cell Biol 30:3126-39
Veldhuis, Johannes D; Keenan, Daniel M; Liu, Peter Y et al. (2009) The aging male hypothalamic-pituitary-gonadal axis: pulsatility and feedback. Mol Cell Endocrinol 299:14-22

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