(Center Overview) Indiana University has over the last twenty years developed a unique, highly interactive, integrated and synergistic team of physicians, basic scientists, computer scientists, engineers and a highly trained and committed technical support team, powered by cutting-edge equipment and technolog, to develop and utilize intravital optical microscopy and 3D quantitative digital image analysis of the kidney. This scientific hub has resulted in a highly productive Division of Nephrology with far-reaching interactions involving multiple investigators on the IU medical school campus, Purdue University, Indiana University- Bloomington and beyond. In particular, the existing O'Brien Center for Advanced Renal Microscopic Analysis has allowed our biomedical base and outside users from around the world to benefit from the scientific expertise and equipment available at the imaging center. The development and user-based customization of specialized techniques of microscopy and quantitative analysis have been a hallmark of the existing P-30 center. Therefore, the primary purpose of this proposal is to continue to offer and further develop an integrated approach to quantitative intravital and tissue microscopy to facilitate molecular, cellular and genetic approaches to the study of disease processes and their therapy to our biomedical base and investigators from around the world. We have added a quantitative 3D tissue imaging core to address the need for specialized expertise and infrastructure to support the collection and quantitative analysis of large-scale, high-content and high resolution microscopy data. In doing so, this Center will provide renal/urological researchers with a novel set of research methods, quantitative analysis tools and fluorescent biosensors or probes not available elsewhere, tools that will facilitate biomedical research, drug discovery, and therapeutic approaches to Kidney and Urologic diseases.

Public Health Relevance

(Center Overview) Research supported by the Indiana NIH-P30 O'Brien Center will continue to advance the understanding of Kidney physiology, and the pathophysiology of disease processes, by developing and using cutting edge optical microscopy techniques and quantitative digital analysis tools in preclinical studies. Paradigm shifting discoveries have and will continue to be made, and the studies will assist in translational studies.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Center Core Grants (P30)
Project #
5P30DK079312-14
Application #
9963197
Study Section
Special Emphasis Panel (ZDK1)
Program Officer
Kimmel, Paul
Project Start
2007-08-03
Project End
2022-06-30
Budget Start
2020-07-01
Budget End
2021-06-30
Support Year
14
Fiscal Year
2020
Total Cost
Indirect Cost
Name
Indiana University-Purdue University at Indianapolis
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
603007902
City
Indianapolis
State
IN
Country
United States
Zip Code
46202
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Winfree, Seth; Dagher, Pierre C; Dunn, Kenneth W et al. (2018) Quantitative Large-Scale Three-Dimensional Imaging of Human Kidney Biopsies: A Bridge to Precision Medicine in Kidney Disease. Nephron 140:134-139
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Swallow, E A; Aref, M W; Chen, N et al. (2018) Skeletal accumulation of fluorescently tagged zoledronate is higher in animals with early stage chronic kidney disease. Osteoporos Int 29:2139-2146
Micanovic, Radmila; Khan, Shehnaz; Janosevic, Danielle et al. (2018) Tamm-Horsfall Protein Regulates Mononuclear Phagocytes in the Kidney. J Am Soc Nephrol 29:841-856
Hato, Takashi; Zollman, Amy; Plotkin, Zoya et al. (2018) Endotoxin Preconditioning Reprograms S1 Tubules and Macrophages to Protect the Kidney. J Am Soc Nephrol 29:104-117
Winfree, Seth; Hato, Takashi; Day, Richard N (2017) Intravital microscopy of biosensor activities and intrinsic metabolic states. Methods 128:95-104
Collett, Jason A; Mehrotra, Purvi; Crone, Allison et al. (2017) Endothelial colony-forming cells ameliorate endothelial dysfunction via secreted factors following ischemia-reperfusion injury. Am J Physiol Renal Physiol 312:F897-F907

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