Strategic Vision and Impact on Environmental Health A. Overview Environmental genomics focuses on the study of the gene-gene and gene-environment interactions that mediate many of the complex diseases affecting human populations in today's world. This field combines the knowledge derived from modern human and molecular genetics with studies of mRNA, protein and metabolite profiling to understand how genes and genetic variations interact with environmental factors to cause disease. Integrative biology is a closely related field focused on the application of systems theory to the study of biological behavior and pathways critical to the preservation of health or the onset and progression of disease. Advances in the fields of environmental genomics and integrative biology during the past five years have been fueled by the need to translate molecular knowledge into concepts that impact our day-to-day lives and that improve the practice of environmental medicine and public health. To this end, University of Louisville (UofL) researchers have implemented academic and research programs in the areas of genetics and molecular medicine leading to the development of a strong infrastructure in DMA sequencing technologies, siRNA repositories, transcriptional profiling, mass-spectrometry (MS)-based protein analysis and proteomics, NMRand MS-based metabolomics, and more recently, bioinformatics. The establishment of a federally-funded Center for Environmental Genomics and Integrative Biology (CEGIB) at UofL will help environmental health researchers with the management and interpretation of data emerging from 'omic technologies and facilitate translation of these findings to public health and clinical practice. Full realization of the benefits offered by molecular understanding of gene, protein and metabolite expression profiles, the molecular circuitry that integrates the biological response to environmental injury, and the identification of novel biomarkers of disease, will require strong infrastructure support in the areas of bioinformatics, biostatistics, computational biology and bench-to-bedside or bench-to-community integration. In this application, we seek funding to create a National Institute of Environmental Health Sciences (NIEHS) Core Center at UofL that focuses on the application of genomics and integrative biology to problems in environmental health. The UofL Center will promote scientific achievements that advance our molecular understanding of human diseases and the role of environmental factors in the pathogenesis of disorders of cellular growth and differentiation. The thematic areas of scientific focus at UofL are environmental cardiology, environmental carcinogenesis and developmental origins of health and disease. These NIEHS-funded areas of scientific activity are ripe for continued expansion and will be greatly facilitated by the centralized infrastructure promoted by CEGIB. NIEHS investments at UofL will be leveraged by outstanding institutional commitments to continue support of core functions in transcriptional profiling, proteomics and metabolomics and to establish clinical and epidemiological research centers. Concerted efforts over the next four years will be directed at the integration of disparate datasets to identify gene, protein and metabolite networks, the development of mathematical models to simulate and predict biological behavior, and the establishment of productive partnerships between basic and clinical researchers that advance research findings in environmental genomics from the bench to the bedside. These efforts will help UofL environmental health science investigators remain competitive and at the cutting edge of biomedical research. Biological complexity is perhaps nothing more than a reflection of the complexities inherent to the natural world. If environmental health researchers are to sustain their impact in advancing the biomedical research agenda in this country, an integrated way of thinking that """"""""marries"""""""" molecular biology to the higher levels of biological organization needs to be nurtured and cultivated among the environmental health research community. In this context, it is important to recognize that systems level thinking in environmental genomics is not restricted to biomedical questions, but that it crosses over to the domains of clinical medicine and public health. Indeed, realization of the potential of 'omic technologies will require creation of structures that support the research enterprise and advance translation of molecular findings to practice. The team of UofL researchers assembled in this application can contribute in meaningful ways to this goal.

National Institute of Health (NIH)
National Institute of Environmental Health Sciences (NIEHS)
Center Core Grants (P30)
Project #
Application #
Study Section
Environmental Health Sciences Review Committee (EHS)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Louisville
United States
Zip Code
Bojang, Pasano; Ramos, Kenneth S (2016) Analysis of LINE-1 Retrotransposition at the Single Nucleus Level. J Vis Exp :
Llorens, M Candelaria; Lorenzatti, Guadalupe; Cavallo, Natalia L et al. (2016) Phosphorylation Regulates Functions of ZEB1 Transcription Factor. J Cell Physiol 231:2205-17
Neal, Rachel E; Chen, Jing; Webb, Cindy et al. (2016) Developmental cigarette smoke exposure II: Hepatic proteome profiles in 6 month old adult offspring. Reprod Toxicol 65:414-424
Montoya-Durango, Diego E; Ramos, Kenneth A; Bojang, Pasano et al. (2016) LINE-1 silencing by retinoblastoma proteins is effected through the nucleosomal and remodeling deacetylase multiprotein complex. BMC Cancer 16:38
Neal, Rachel E; Jagadapillai, Rekha; Chen, Jing et al. (2016) Developmental cigarette smoke exposure II: Kidney proteome profile alterations in 6 month old adult offspring. Reprod Toxicol 65:425-435
Xu, Xin; Prough, Russell A; Samuelson, David J (2015) Differential 12-O-Tetradecanoylphorbol-13-acetate-induced activation of rat mammary carcinoma susceptibility Fbxo10 variant promoters via a PKC-AP1 pathway. Mol Carcinog 54:134-47
Al-Eryani, Laila; Wahlang, Banrida; Falkner, K C et al. (2015) Identification of Environmental Chemicals Associated with the Development of Toxicant-associated Fatty Liver Disease in Rodents. Toxicol Pathol 43:482-97
Gordon, Michael W; Yan, Fang; Zhong, Xiaoming et al. (2015) Regulation of p53-targeting microRNAs by polycyclic aromatic hydrocarbons: Implications in the etiology of multiple myeloma. Mol Carcinog 54:1060-9
Bamji, Sanaya F; Page, Robert B; Patel, Dharti et al. (2015) Soy glyceollins regulate transcript abundance in the female mouse brain. Funct Integr Genomics 15:549-61
Ramos, Irma N; Appana, Savitri N; Brock, Guy et al. (2015) Health status, perceptions and needs of Hispanics in rural Shelbyville, Kentucky. J Immigr Minor Health 17:148-55

Showing the most recent 10 out of 183 publications