The two major functions of the Cell Culture Module are (1) to help investigators use cultures effectively in their research, and (2) to provide a mechanism for optimum sharing of ocular specimens from human and animal eyes. To fulfill the first function, the Module: (a) serves as a repository for several types of cultured ocular cells and cell lines, (b) provides a centralized facility with shared-use equipment for culture maintenance for those who lack culture facilities in their own labs, (c) provides some shareduse instruments (such as inverted phase and fluorescence photomicroscopes) for investigators who have basic culture facilities to avoid duplication of all equipment, and (d) assists investigators in using in vitro models for their work including bringing culture methods into their own labs by training laboratory personnel in culture techniques. The availability of this Module also improves the economy of our lab operations since common-use culture supplies can be purchased in bulk.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY001931-32
Application #
7587277
Study Section
Special Emphasis Panel (ZEY1)
Project Start
Project End
Budget Start
2008-04-01
Budget End
2009-03-31
Support Year
32
Fiscal Year
2008
Total Cost
$166,284
Indirect Cost
Name
Medical College of Wisconsin
Department
Type
DUNS #
937639060
City
Milwaukee
State
WI
Country
United States
Zip Code
53226
Hirji, Nashila; Georgiou, Michalis; Kalitzeos, Angelos et al. (2018) Longitudinal Assessment of Retinal Structure in Achromatopsia Patients With Long-Term Follow-up. Invest Ophthalmol Vis Sci 59:5735-5744
Krawitz, Brian D; Phillips, Erika; Bavier, Richard D et al. (2018) Parafoveal Nonperfusion Analysis in Diabetic Retinopathy Using Optical Coherence Tomography Angiography. Transl Vis Sci Technol 7:4
Sajdak, Benjamin S; Bell, Brent A; Lewis, Tylor R et al. (2018) Assessment of Outer Retinal Remodeling in the Hibernating 13-Lined Ground Squirrel. Invest Ophthalmol Vis Sci 59:2538-2547
Datta, Shyamtanu; Renwick, Marian; Chau, Viet Q et al. (2018) A Destabilizing Domain Allows for Fast, Noninvasive, Conditional Control of Protein Abundance in the Mouse Eye - Implications for Ocular Gene Therapy. Invest Ophthalmol Vis Sci 59:4909-4920
Mainali, Laxman; O'Brien, William J; Subczynski, Witold K (2018) Detection of cholesterol bilayer domains in intact biological membranes: Methodology development and its application to studies of eye lens fiber cell plasma membranes. Exp Eye Res 178:72-81
Hendee, Kathryn E; Sorokina, Elena A; Muheisen, Sanaa S et al. (2018) PITX2 deficiency and associated human disease: insights from the zebrafish model. Hum Mol Genet 27:1675-1695
Lapierre-Landry, Maryse; Huckenpahler, Alison L; Link, Brian A et al. (2018) Imaging Melanin Distribution in the Zebrafish Retina Using Photothermal Optical Coherence Tomography. Transl Vis Sci Technol 7:4
Patterson, Emily J; Kalitzeos, Angelos; Kasilian, Melissa et al. (2018) Residual Cone Structure in Patients With X-Linked Cone Opsin Mutations. Invest Ophthalmol Vis Sci 59:4238-4248
Davidson, Benjamin; Kalitzeos, Angelos; Carroll, Joseph et al. (2018) Automatic Cone Photoreceptor Localisation in Healthy and Stargardt Afflicted Retinas Using Deep Learning. Sci Rep 8:7911
Reid, Christopher A; Nettesheim, Emily R; Connor, Thomas B et al. (2018) Development of an inducible anti-VEGF rAAV gene therapy strategy for the treatment of wet AMD. Sci Rep 8:11763

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