The Tissue Culture/Hybridoma/Molecular Module, located in the Department of Anatomy/Cell Biology will provide a centralized facility for tissue and organ culture and an experienced research assistant. The research assistant will be available to help investigators who have little expertise in tissue culture to develop experimental methods relevant to their studies. In addition, she will assist investigators with the production of mAbs and in carrying out routine molecular techniques. Projects proposed requiring Module use will be presented to the Module co-director (Dr. Kurpakus-Wheater) for initial consultation. Once approved, the projects will be undertaken by either the PI proposing the work or personnel from his/her laboratory under direct supervision and with the help of the Module research assistant. The newly dedicated space and equipment for molecular studies has greatly expanded and enhanced the functions of this module. Those investigators who lack experience in the technology required for molecular biology, tissue/organ culture, or mAb production will rely heavily on the equipment and availability of the research assistant for technical advice to their projects. Those investigators knowledgeable in the technical aspects involved will use the facility to enhance their own studies and to begin pilot research projects. This centralized Core facility will thus continue to provide a reasonable, cost efficient mechanism to facilitate and enhance numerous NEI funded research projects.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY004068-23
Application #
7553177
Study Section
Project Start
Project End
Budget Start
2005-04-01
Budget End
2006-03-31
Support Year
23
Fiscal Year
2005
Total Cost
$92,595
Indirect Cost
Name
Wayne State University
Department
Type
DUNS #
001962224
City
Detroit
State
MI
Country
United States
Zip Code
48202
Berkowitz, Bruce A (2018) Oxidative stress measured in vivo without an exogenous contrast agent using QUEST MRI. J Magn Reson 291:94-100
Shi, Haoshen; Ebrahim, Abdul S; Berger, Elizabeth A (2018) A Contrast in Pathogenic Responses between C57BL/6J and BALB/cJ Mice Using a Model of Retinal Injury. Am J Pathol 188:2717-2728
Curtiss, Elizabeth; Liu, Li; Steinle, Jena J (2018) miR15a regulates NLRP3 inflammasome proteins in the retinal vasculature. Exp Eye Res 176:98-102
Gao, Nan; Me, Rao; Dai, Chenyang et al. (2018) Opposing Effects of IL-1Ra and IL-36Ra on Innate Immune Response to Pseudomonas aeruginosa Infection in C57BL/6 Mouse Corneas. J Immunol 201:688-699
Curtiss, Elizabeth; Jiang, Youde; Liu, Li et al. (2018) Epac1 Restores Normal Insulin Signaling through a Reduction in Inflammatory Cytokines. Mediators Inflamm 2018:3809092
Singh, Pawan Kumar; Khatri, Indu; Jha, Alokkumar et al. (2018) Determination of system level alterations in host transcriptome due to Zika virus (ZIKV) Infection in retinal pigment epithelium. Sci Rep 8:11209
Han, Jing; Li, Yue; Liu, Xiuli et al. (2018) Metformin suppresses retinal angiogenesis and inflammation in vitro and in vivo. PLoS One 13:e0193031
Berkowitz, Bruce A; Podolsky, Robert H; Berri, Ali M et al. (2018) Dark Rearing Does Not Prevent Rod Oxidative Stress In Vivo in Pde6brd10 Mice. Invest Ophthalmol Vis Sci 59:1659-1665
Jiang, Youde; Liu, Li; Steinle, Jena J (2018) miRNA15a regulates insulin signal transduction in the retinal vasculature. Cell Signal 44:28-32
Liu, Li; Patel, Paragi; Steinle, Jena J (2018) PKA regulates HMGB1 through activation of IGFBP-3 and SIRT1 in human retinal endothelial cells cultured in high glucose. Inflamm Res 67:1013-1019

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