Continuing funds are requested by a group of 20 vision scientists and their laboratory personnel to support two research modules housed in the Department of Anatomy/Cell Biology that will continue to provide central units that will facilitate and enhance vision research at Wayne State and other participating Universities lacking core supported facilities. The modules will cost effectively enhance current research by providing this group of scientists with personnel and equipment, often not supported by individual R01 or other types of NEI grants, to advance NEI-funded research as a priority and in addition to provide opportunity for collaboration and for new pilot project development leading to increased NEI grant submissions. The two modules requested include: Imaging/Histopathology and Tissue Culture/Molecular. The Imaging/ Histopathology module provides for all facets of confocal laser scanning, light and electron microscopy, immunofluorescence, image analysis using metamorph imaging software, as well as training on microscopes and imaging software. There is assistance and training in the management of digital images, including digital graphics production, animal slit lamp photography, poster and publication production, digital photography equipment use, high resolution flatbed transparency scanning and imaging computer workstation usage within the module. The Tissue Culture/Molecular module provides assistance and training for preparation of specialized media, isolation, purification and characterization of ocular cells for primary culture, subculture and propagation of established cell lines, and cryopreservation of cells. Assistance and training is also available for fundamental techniques in molecular biology including real time RT-PCR analysis, quantification and imaging of gels and storage of bacteria, vectors and cDNA constructs. Each of the modules is staffed by well-trained research assistants who are knowledgeable in the diverse areas of expertise required for each facility. The directorship of each module is composed of established vision scientists who have considerable expertise in the respective research field and who actively function to enhance NEI-supported studies, and develop, encourage and facilitate collaborative efforts in the visual sciences.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
5P30EY004068-30
Application #
8244497
Study Section
Special Emphasis Panel (ZEY1-VSN (04))
Program Officer
Liberman, Ellen S
Project Start
1997-04-01
Project End
2014-03-31
Budget Start
2012-04-01
Budget End
2014-03-31
Support Year
30
Fiscal Year
2012
Total Cost
$429,214
Indirect Cost
$144,022
Name
Wayne State University
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
001962224
City
Detroit
State
MI
Country
United States
Zip Code
48202
Han, Jing; Li, Yue; Liu, Xiuli et al. (2018) Metformin suppresses retinal angiogenesis and inflammation in vitro and in vivo. PLoS One 13:e0193031
Berkowitz, Bruce A; Podolsky, Robert H; Berri, Ali M et al. (2018) Dark Rearing Does Not Prevent Rod Oxidative Stress In Vivo in Pde6brd10 Mice. Invest Ophthalmol Vis Sci 59:1659-1665
Jiang, Youde; Liu, Li; Steinle, Jena J (2018) miRNA15a regulates insulin signal transduction in the retinal vasculature. Cell Signal 44:28-32
Liu, Li; Patel, Paragi; Steinle, Jena J (2018) PKA regulates HMGB1 through activation of IGFBP-3 and SIRT1 in human retinal endothelial cells cultured in high glucose. Inflamm Res 67:1013-1019
Carion, Thomas W; Greenwood, Matthew; Ebrahim, Abdul Shukkur et al. (2018) Immunoregulatory role of 15-lipoxygenase in the pathogenesis of bacterial keratitis. FASEB J 32:5026-5038
Shi, Haoshen; Berger, Elizabeth A (2018) Characterization of Site-Specific Phosphorylation of NF-?B p65 in Retinal Cells in Response to High Glucose and Cytokine Polarization. Mediators Inflamm 2018:3020675
Guest, John M; Singh, Pawan Kumar; Revankar, Sanjay G et al. (2018) Isavuconazole for Treatment of Experimental Fungal Endophthalmitis Caused by Aspergillus fumigatus. Antimicrob Agents Chemother 62:
Berkowitz, Bruce A; Podolsky, Robert H; Qian, Haohua et al. (2018) Mitochondrial Respiration in Outer Retina Contributes to Light-Evoked Increase in Hydration In Vivo. Invest Ophthalmol Vis Sci 59:5957-5964
Cui, Xinhan; Gao, Nan; Me, Rao et al. (2018) TSLP Protects Corneas From Pseudomonas aeruginosa Infection by Regulating Dendritic Cells and IL-23-IL-17 Pathway. Invest Ophthalmol Vis Sci 59:4228-4237
Lu, Qi; Ganjawala, Tushar H; Hattar, Samer et al. (2018) A Robust Optomotor Assay for Assessing the Efficacy of Optogenetic Tools for Vision Restoration. Invest Ophthalmol Vis Sci 59:1288-1294

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