We will purchase two Zeiss 510 Meta confocal microscopes for our studies. These microscopes will be configured on fixed-stage upright microscopes and will come with 458, 477, 488, 5t4, 543 and 633 nm laser lines, two internal photomultiplier tubes and a Meta, 32 element spectral detector. The 510 Meta confocal microscope is the instrument of choice for studies because of its ability to detect emission spectra and to unmix overlapping spectra of multiple indicators while performing the high-quality confocal imaging expected of a 510 system. We feel that the Meta detector is a revolutionary concept in microscopy which will significantly facilitate our biological studies; it will permit our investigators to simultaneously express multiple fluorescent proteins in cells and, despite their similarity in emission spectra, to quantitatively identify the contribution of each. With 20 investigators and 34 NINDS projects in this proposed Center, each investigator would have only two hours per week (or each N1NDS project would have just over one hour per week) on the microscope were we to have only one two photon microscope available. Thus the purchase of two of these two-photon microscopes is essential. Because CHOP appreciates the great expense of these microscopes, it has provided $259,835 to alleviate some of the financial burden.

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS047321-04
Application #
7554026
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2006-08-01
Budget End
2007-07-31
Support Year
4
Fiscal Year
2006
Total Cost
$177,039
Indirect Cost
Name
Children's Hospital of Philadelphia
Department
Type
DUNS #
073757627
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Yu, Esther P; Dengler, Christopher G; Frausto, Shanti F et al. (2013) Protracted postnatal development of sparse, specific dentate granule cell activation in the mouse hippocampus. J Neurosci 33:2947-60
Takano, Hajime; McCartney, Melissa; Ortinski, Pavel I et al. (2012) Deterministic and stochastic neuronal contributions to distinct synchronous CA3 network bursts. J Neurosci 32:4743-54
Zhang, Junxian; Scherer, Steven S; Yum, Sabrina W (2011) Dominant Cx26 mutants associated with hearing loss have dominant-negative effects on wild type Cx26. Mol Cell Neurosci 47:71-8
Yum, Sabrina W; Zhang, Junxian; Scherer, Steven S (2010) Dominant connexin26 mutants associated with human hearing loss have trans-dominant effects on connexin30. Neurobiol Dis 38:226-36
D'Ascenzo, Marcello; Fellin, Tommaso; Terunuma, Miho et al. (2007) mGluR5 stimulates gliotransmission in the nucleus accumbens. Proc Natl Acad Sci U S A 104:1995-2000
Halassa, Michael M; Fellin, Tommaso; Takano, Hajime et al. (2007) Synaptic islands defined by the territory of a single astrocyte. J Neurosci 27:6473-7
Yum, Sabrina W; Zhang, Junxian; Valiunas, Virginijus et al. (2007) Human connexin26 and connexin30 form functional heteromeric and heterotypic channels. Am J Physiol Cell Physiol 293:C1032-48
Pan, Zongming; Kao, Tingching; Horvath, Zsolt et al. (2006) A common ankyrin-G-based mechanism retains KCNQ and NaV channels at electrically active domains of the axon. J Neurosci 26:2599-613
Barrett, Lindy E; Sul, Jai-Yoon; Takano, Hajime et al. (2006) Region-directed phototransfection reveals the functional significance of a dendritically synthesized transcription factor. Nat Methods 3:455-60
Zhang, Guixin; Jin, Li-Qing; Sul, Jai-Yoon et al. (2005) Live imaging of regenerating lamprey spinal axons. Neurorehabil Neural Repair 19:46-57

Showing the most recent 10 out of 11 publications