Abstract

The proposed core facility will include a single large room . It will be used for the analysis of the levels of experimental compounds in various biofluids (plasma, CSF, urine) and tissues (brain, spinal cord) in normal or injured rats or mice. For this purpose, we will transfer the Shimadzu HPLC with ESA Coulichem electrochemical detector to the Core. However, a new state of the art HPLC system with autosampler and electrochemical, UV/Vis, fluorescence and light scattering detectors is requested in the grant budget for year 4. Secondly, the core will be used for carrying out the bioanalytical work associated with exploration of various pathophysiological biomarkers. A major focus will be on the measurement of isoprostanes using GC-MS. The recently purchased Shimadzu GC-MS will be moved to this facility. A microplate reader for protein assays and ELISAs that will be moved to this core. In addition, we will continue working on the potential of CSF alpha-spectrin measurements carried out by immunoblot or ELISA. Most of the latter will take place in Dr. Hall's laboratory space outside of the core. In addition, all animal surgeries, injury procedures and sample collection will take place in the Animal Surgery and TBI/SCI Model Core facility. The Pharmacokinetics & Biomarker Core will only be used for bioanalytical work.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS051220-03
Application #
7439098
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2007-05-01
Budget End
2008-04-30
Support Year
3
Fiscal Year
2007
Total Cost
$82,804
Indirect Cost

  Institution

Name
University of Kentucky
Department
Type
DUNS #
939017877
City
Lexington
State
KY
Country
United States
Zip Code
40506

  Publications

Keeney, Jeriel T R; Ren, Xiaojia; Warrier, Govind et al. (2018) Doxorubicin-induced elevated oxidative stress and neurochemical alterations in brain and cognitive decline: protection by MESNA and insights into mechanisms of chemotherapy-induced cognitive impairment (""chemobrain""). Oncotarget 9:30324-30339
Sama, Diana M; Carlson, Shaun W; Joseph, Binoy et al. (2018) Assessment of systemic administration of PEGylated IGF-1 in a mouse model of traumatic brain injury. Restor Neurol Neurosci 36:559-569
Lanzillotta, Chiara; Tramutola, Antonella; Meier, Shelby et al. (2018) Early and Selective Activation and Subsequent Alterations to the Unfolded Protein Response in Down Syndrome Mouse Models. J Alzheimers Dis 62:347-359
Patel, Samir P; Cox, David H; Gollihue, Jenna L et al. (2017) Pioglitazone treatment following spinal cord injury maintains acute mitochondrial integrity and increases chronic tissue sparing and functional recovery. Exp Neurol 293:74-82
Gensel, John C; Kopper, Timothy J; Zhang, Bei et al. (2017) Predictive screening of M1 and M2 macrophages reveals the immunomodulatory effectiveness of post spinal cord injury azithromycin treatment. Sci Rep 7:40144
Orr, Michael B; Simkin, Jennifer; Bailey, William M et al. (2017) Compression Decreases Anatomical and Functional Recovery and Alters Inflammation after Contusive Spinal Cord Injury. J Neurotrauma 34:2342-2352
Kulbe, Jacqueline R; Hall, Edward D (2017) Chronic traumatic encephalopathy-integration of canonical traumatic brain injury secondary injury mechanisms with tau pathology. Prog Neurobiol 158:15-44
Gollihue, Jenna L; Patel, Samir P; Mashburn, Charlie et al. (2017) Optimization of mitochondrial isolation techniques for intraspinal transplantation procedures. J Neurosci Methods 287:1-12
Hill, Rachel L; Singh, Indrapal N; Wang, Juan A et al. (2017) Time courses of post-injury mitochondrial oxidative damage and respiratory dysfunction and neuronal cytoskeletal degradation in a rat model of focal traumatic brain injury. Neurochem Int 111:45-56
Zhang, Bei; Bailey, William M; McVicar, Anna Leigh et al. (2016) Age increases reactive oxygen species production in macrophages and potentiates oxidative damage after spinal cord injury. Neurobiol Aging 47:157-167

Showing the most recent 10 out of 84 publications