We are constantly looking to employ new methods to enhance the separation and identification of peptides. We are fortunate to have Dr. David Goodlett as a core director as he is actively involved in the development of new mass spectrometry techniques (please see his biosketch and letter). We have also recruited an internationally recognized expert on mass spectrometry and bioinformatics, Dr. Timothy Veenstra, as an external consultant. These two individuals will insure that Core C provides state of the art advances to our end users. One future direction will involve incorporating stable isotope labeling methods, e.g. isotope coded affinity tag (ICAT) or iTRAQ methods, as well as so-called label-free methods as recently described in Dr. Goodlett's laboratory (Foss et al., In Press, Nature Genetics). This will allow us to provide information on the relative levels of expression of proteins in a complex and discern bona fide members of the complexes from non-specific, contaminating proteins58. In addition, depending on our resources, it may be possible to provide global proteome analysis as we previously described for mouse cortical neurons57 and mouse astrocytes (McBee et al, In Press, Proteomics;see Morrison Biosketch).

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS055088-04
Application #
8374484
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2011-12-01
Budget End
2012-11-30
Support Year
4
Fiscal Year
2012
Total Cost
$272,568
Indirect Cost
$97,845
Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195
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