We are constantly looking to employ new methods to enhance the separation and identification of peptides. We are fortunate to have Dr. David Goodlett as a core director as he is actively involved in the development of new mass spectrometry techniques (please see his biosketch and letter). We have also recruited an internationally recognized expert on mass spectrometry and bioinformatics, Dr. Timothy Veenstra, as an external consultant. These two individuals will insure that Core C provides state of the art advances to our end users. One future direction will involve incorporating stable isotope labeling methods, e.g. isotope coded affinity tag (ICAT) or iTRAQ methods, as well as so-called label-free methods as recently described in Dr. Goodlett's laboratory (Foss et al., In Press, Nature Genetics). This will allow us to provide information on the relative levels of expression of proteins in a complex and discern bona fide members of the complexes from non-specific, contaminating proteins58. In addition, depending on our resources, it may be possible to provide global proteome analysis as we previously described for mouse cortical neurons57 and mouse astrocytes (McBee et al, In Press, Proteomics;see Morrison Biosketch).
| Meabon, James S; de Laat, Rian; Ieguchi, Katsuaki et al. (2016) Intracellular LINGO-1 negatively regulates Trk neurotrophin receptor signaling. Mol Cell Neurosci 70:1-10 |
| Yen, Gloria S; Edgar, J Scott; Yoon, Sung Hwan et al. (2016) Polydimethylsiloxane microchannel coupled to surface acoustic wave nebulization mass spectrometry. Rapid Commun Mass Spectrom 30:1096-100 |
| Young, Jennifer M; Nelson, Jonathan W; Cheng, Jian et al. (2015) Peroxisomal biogenesis in ischemic brain. Antioxid Redox Signal 22:109-20 |
| Wang, David B; Kinoshita, Yoshito; Kinoshita, Chizuru et al. (2015) Loss of endophilin-B1 exacerbates Alzheimer's disease pathology. Brain 138:2005-19 |
| Mikheev, Andrei M; Mikheeva, Svetlana A; Trister, Andrew D et al. (2015) Periostin is a novel therapeutic target that predicts and regulates glioma malignancy. Neuro Oncol 17:372-82 |
| Wang, David B; Kinoshita, Chizuru; Kinoshita, Yoshito et al. (2014) p53 and mitochondrial function in neurons. Biochim Biophys Acta 1842:1186-97 |
| Wang, David B; Uo, Takuma; Kinoshita, Chizuru et al. (2014) Bax interacting factor-1 promotes survival and mitochondrial elongation in neurons. J Neurosci 34:2674-83 |
| Wang, David B (2013) Monophosphoryl lipid A is an lipopolysaccharide-derived Toll-like receptor 4 agonist which may improve Alzheimer's disease pathology. Expert Opin Biol Ther 13:1639-41 |
| Wang, David B; Garden, Gwenn A; Kinoshita, Chizuru et al. (2013) Declines in Drp1 and parkin expression underlie DNA damage-induced changes in mitochondrial length and neuronal death. J Neurosci 33:1357-65 |
| Mikheev, Andrei M; Ramakrishna, Rohan; Stoll, Elizabeth A et al. (2012) Increased age of transformed mouse neural progenitor/stem cells recapitulates age-dependent clinical features of human glioma malignancy. Aging Cell 11:1027-35 |
Showing the most recent 10 out of 13 publications
